Programmed cell death 1 ligand 1 (PDL-1) is a B7 related protein belonging to the BTN/MOG family. PDL-1 is essential for T-cell proliferation and production of IL10 and IFNG. PDL-1 reduces the secretion of IL-2 and IL-10 from memory T cells by inhibiting cell-mediated immune responses. PDL-1 may be involved in reducing allogenic CD4+ memory T-cell responses to endothelial cells. PDL-1 is highly expressed in heart, skeletal muscle, placenta, and lung and weakly expressed in thymus, spleen, kidney, and liver.

Epitope: extracellular domain

GST-tagged recombinant protein corresponding to the extracellular domain of human PDL-1/CD274.

Anti-PDL-1/CD274 Antibody, clone 3F2.1 is an antibody against PDL-1/CD274 for use in western blotting & IHC.

Immunohistochemistry Analysis: A 1:50 and 1:500 dilution from a representative lot detected PDL-1/CD274 in human tonsil and skeletal muscle tissue, respectively.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Evaluated by Western Blotting in human skeletal muscle tissue.

Western Blotting Analysis: 1 µg/mL of this antibody detected PDL-1/CD274 in 10 µg of human skeletal muscle tissue.

~40 kDa observed. The calculated molecular weight is 33 kDa; however, this protein may be observed at higher molecular weights to due to heavy glycosylation.

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.