MABN495

Anti-Aldh1L1 Antibody, clone N103/39

clone N103/39, from mouse

• Synonym(s): Cytosolic 10-formyltetrahydrofolate dehydrogenase, 10-FTHFDH, FDH, Aldehyde dehydrogenase family 1 member L1 ; ; FBP-CI
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: N103/39, monoclonal
• species reactivity: human, rat, mouse
• technique(s): immunofluorescence: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_071992
• UniProt accession no.: P28037

Description

General description

Aldh1L1, also known as 10-formyltetrahydrofolate dehydrogenase (10-FTHFDH), is a multidomain protein that is found in liver cytosol and also serves as a CNS astrocyte marker. Aldh1L1 is comprised of two functionally unrelated domains, an aldehyde dehydrogenase-homologous domain and a folate-binding hydrolase domain. These two domains are thought to be connected by a 100-residue linker. It is known to induce phosphorylation of p53 at Ser6, which is a critical step in stimulating apoptosis.

Specificity

This Aldh1L1 monoclonal antibody does not exhibit cross-reactivity toward Aldh1L2.

Immunogen

Recombinant full-length rat Aldh1L1.

Application

Anti-Aldh1L1, clone N103/39 is an antibody targeting the Aldh1L1 protein, validated for use in Immunofluorescence, Immunohistochemistry, and Western Blotting.

Research Category
Neuroscience

Research Sub Category
Sensory & PNS

Western Blot Analysis: 0.5 µg/mL from a representative lot detected Aldh1L1 in 10 µg of human brain tissue lysate.

Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected Aldh1L1 rat cerebral cortex tissue and human pons/midbrain tissue.

Immunofluorescence Analysis: A representative lot detected Aldh1L1 in rat cortex and cerebellum tissue.

Quality

Evaluated by Western Blot in mouse brain tissue lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Aldh1L1 in 10 µg of mouse brain tissue lysate.

Target description

~99 kDa observed

Physical form

Format: Purified

Protein G Purified

Purified mouse IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Mouse brain tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable