Low-Density Lipoprotein (LDL) Receptor is a mosaic protein that mediates the endocytosis of cholesterol-rich LDL. It is a cell-surface receptor that recognizes the apoprotein B100 which is embedded in the phospholipid outer layer of LDL particles. The receptor also recognizes the apoE protein found in chylomicron remnants and VLDL remnants (IDL). Mutations in LDLR are associated with familial hypercholesterolemia which is characterized by high levels of LDL in the blood.

This antibody recognizes LDLR.

Partially purified bovine adrenal LDL receptor

Research Category
Metabolism

Research Sub Category
Lipid Metabolism & Weight Regulation

This Anti-LDLR Antibody, clone C7 is validated for use in WB for the detection of LDLR.

Evaluated by Western Blot in ARPE-19 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected LDLR on 10 µg of ARPE-19 cell lysate.

160 kDa Observed The translation of LDLR mRNA yields a 120 kDa protein that is post-translationally modified in the Golgi apparatus into the mature, 160 kDa LDLR. (Tolleshaug, H., et al. (1982). Cell 30:715–724.)

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
ARPE-19 cell lysate

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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