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SCR103

Sigma-Aldrich

Collagenase type I

The collagenase type I (from Clostridium histolyticum) is a crude collagenase preparation that can be used for the isolation of primary cells or for tissue dissociation by enzymatic means.

Synonym(s):

Collagenase Type I from Clostridium histolyticum, Tissue dissociation

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1 VIAL
CA$1,100.00

CA$1,100.00

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1 VIAL
CA$1,100.00

About This Item

UNSPSC Code:
12352204
eCl@ss:
32160801
NACRES:
NA.75

CA$1,100.00

Please contact Customer Service for Availability
Request a Bulk Order
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Quality Level

100

form

powder

manufacturer/tradename

Chemicon®

technique(s)

cell culture | stem cell: suitable

input

sample type induced pluripotent stem cell(s)
sample type: human embryonic stem cell(s)

shipped in

wet ice

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This Item
P6993C4237M1941
Protein Phosphatase 2A2 from bovine kidney buffered aqueous glycerol solution

P1868

Protein Phosphatase 2A2 from bovine kidney

Protein Phosphatase 2A1 bovine ≥1500 units/mg protein

P6993

Protein Phosphatase 2A1 bovine

Protamine Kinase, Cytosolic from bovine kidney >90% (SDS-PAGE), buffered aqueous glycerol solution

C4237

Protamine Kinase, Cytosolic from bovine kidney

Myelin Basic Protein Kinase 2 from bovine kidney >90% (SDS-PAGE)

M1941

Myelin Basic Protein Kinase 2 from bovine kidney

specific activity

~2.0 U/vial

specific activity

≥1500 units/mg protein

specific activity

≥15,000 units/mg protein

specific activity

≥1000 units/mg protein

biological source

bovine kidney

biological source

bovine

biological source

-

biological source

bovine kidney

form

buffered aqueous glycerol solution

form

solution

form

buffered aqueous glycerol solution

form

solution

mol wt

dimer 101 kDa (65 kDa and 36 kDa)

mol wt

192 kDa

mol wt

45 kDa

mol wt

40 kDa

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

General description

Collagenase type I (from Clostridium histolyticum) is a crude collagenase preparation that can be used for the isolation of primary cells or for tissue dissociation by enzymatic means. The preparation also may contain caseinase, clostripain, and tryptic activities. This preparation can be used to dissociate cells in culture, and has been successfully used to passage human embryonic stem cells cultured with Millipore’s HEScGROTM Animal Component-Free Medium.

Packaging

1 vial containing 250mg of lyophilized powder

Quality

Each lot of collagenase type I is assayed via a modification of Mandl (1964), with the extent of collagen breakdown determined using the colorimetric ninhydrin method of Moore and Stein (1948). Each lot is then tested for successful passage of human embryonic stem cells cultured in Millipore′s HEScGRO Medium for hES cells. Cells are characterized for correct marker expression and morphology after five passages.

Physical form

Collagenase type I is supplied as a dialyzed, lyophilized powder.

Storage and Stability

Collagenase type I should be stored in its powdered form at 2-8oC until ready to use. Solutions of collagenase type I can be aliquotted and stored at -20oC, but should not be repeatedly thawed and re-frozen.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Danger

Hazard Statements

H315,H319,H334,H335

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

  • Specification Sheet

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    Protein Phosphatase 2A is a specific protamine-kinase-inactivating phosphatase.
    Amick, G.D, et al.
    The Biochemical Journal, (Pt 3), 1019-1022 (1992)
    G D Amick et al.
    Archives of biochemistry and biophysics, 297(1), 80-85 (1992-08-15)
    About an eightfold increase in protamine kinase activity was detected following extraction of highly purified microsomes from bovine kidney with 1% Triton X-100. Relative to the soluble fraction, the microsomes contained about 30% protamine kinase activity. The microsomal protamine kinase
    H R Matthews et al.
    FEBS letters, 364(1), 51-54 (1995-05-01)
    Whole cell extracts from rat liver or spinach leaves contain divalent ion-independent protein histidine phosphatase activity due to phosphatases of the PP1/PP2A family. In the rat liver extract, almost all the activity was found in the PP1, PP2A1 and PP2A2
    M Li et al.
    Biochemistry, 34(6), 1988-1996 (1995-02-14)
    Two heat-stable protein inhibitors of protein phosphatase 2A (PP2A), tentatively designated I1PP2A and I2PP2A, have been purified to apparent homogeneity from extracts of bovine kidney. The purified preparations of I1PP2A exhibited an apparent M(r) approximately 30,000 and 250,000 as determined
    A Hiraga et al.
    The Biochemical journal, 346 Pt 2, 433-439 (2000-03-24)
    Protein phosphatase (PP) 2A1, a trimer composed of A-, B- and C-subunits in the PP2A family, has been regarded as a principal form localizing at microtubules (MT), but PP2A2, the dimer of A- and C-subunits, has not. Substantiating the claim
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