08062

Protein G′ from Streptococcus sp.

recombinant, expressed in E. coli

• Synonym(s): IgG F(c)-Receptor
• MDL number: MFCD00132110
• UNSPSC Code: 12352202
• NACRES: NA.26

Properties

• biological source: bacterial (Streptococcus sp.)
• Quality Level: 100
• recombinant: expressed in E. coli
• form: lyophilized solid
• mol wt: ~30 kDa
• capacity: ~5 mg/mg, solid binding capacity (IgG)
• storage temp.: −20°C

Description

General description

Genetically engineered truncated protein G; retains affinity for IgG, but lacks albumin and Fab binding sites and membrane-binding regions.

Protein G, a cell wall protein, is obtained from group G streptococci. The extracellular part of this protein is made of two/three small domains or serum albumin binding (GA domains) and two/three immunoglobulin (IgG) binding domains (B domains).

Application

Protein G′ from Streptococcus sp. has been used in in vitro actin labeling assay.

Biochem/physiol Actions

Protein G can bind all the human and mouse IgG subclasses. It can bind to both the fragment crystallizable (Fc) and antigen-binding fragment (Fab) components of the antibody.

Physical form

lyophilized powder in a Tris-HCl buffer, pH 7.5

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves