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22244

Sigma-Aldrich

Ceramide from bovine spinal cord

≥98.0% (TLC)

Synonym(s):

Bovine Spinal Cord Ceramide

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96 WELLS
RM 3,052.00

About This Item

MDL number:
MFCD00146359
UNSPSC Code:
12352211
NACRES:
NA.25

RM 3,052.00

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biological source

bovine spinal cord

Quality Level

100

Assay

≥98.0% (TLC)

form

solid

impurities

α-hydroxy fatty acids

solubility

chloroform/methanol (99:1): 20 mg/mL, clear, colorless

lipid type

sphingolipids

shipped in

wet ice

storage temp.

−20°C

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Gene Information

human ... CES2(8824)

Gene Information

human ... BIRC2(329)

Gene Information

human ... EPHB3(2049)

Gene Information

human ... FLRT2(23768)

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

human

input

sample type plasma
sample type cell culture supernatant(s)
sample type serum

input

sample type cell culture supernatant(s)
sample type serum
sample type plasma

input

sample type serum
sample type cell culture supernatant(s)
sample type plasma

input

sample type serum
sample type plasma
sample type cell culture supernatant(s)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 1.6 ng/mL
standard curve range: 1.638-400 ng/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 1.65 ng/mL
standard curve range: 1.638-400 ng/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 1.8 ng/mL
standard curve range: 1.64-400 ng/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 1.6 ng/mL
standard curve range: 1.638-400 ng/mL

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

Application

Ceramide is used to investigate effects on programmed cell death (apoptosis) and insulin signaling.

Biochem/physiol Actions

Ceramide is a minor component of cell membranes. Activates atypical protein kinase C (e.g., PKCzeta) [1].

Preparation Note

from cerebrosides by a modification of the procedure by H.E. Carter et al., J. Lipid Res. 2, 228 (1961)

Other Notes

Sales restrictions may apply

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
SLCL1004
SLCH6735
SLCF0412
SLBX4561
SLBS2960V

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