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P5400

Sigma-Aldrich

Pectinesterase from orange peel

greener alternative

lyophilized powder, ≥150 units/mg protein

Synonym(s):

Pectin methylesterase, Pectin pectylhydrolase

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1000 UNITS
$1,270.00

About This Item

CAS Number:
9025-98-3
EC Number:
3.1.1.11 (BRENDA, IUBMB)
EC Number:
232-807-0
MDL number:
MFCD00131812
UNSPSC Code:
12352204
NACRES:
NA.54

$1,270.00

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form

lyophilized powder

Quality Level

200

specific activity

≥150 units/mg protein

composition

Protein, 20-50% biuret

greener alternative product characteristics

Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

greener alternative category

, Enabling

storage temp.

2-8°C

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P5936P2401C1794
Pectinesterase from orange peel lyophilized powder, ≥150 units/mg protein

P5400

Pectinesterase from orange peel

Pectolyase from Aspergillus japonicus suitable for plant cell culture, lyophilized powder

P5936

Pectolyase from Aspergillus japonicus

Pectinase from Rhizopus sp. powder, 400-800 units/g solid

P2401

Pectinase from Rhizopus sp.

Cellulase from Trichoderma sp. BioReagent, suitable for plant cell culture, 3-10 units/mg solid

C1794

Cellulase from Trichoderma sp.

specific activity

≥150 units/mg protein

specific activity

≥0.3 unit/mg solid

specific activity

400-800 units/g solid

specific activity

3-10 units/mg solid

form

lyophilized powder

form

lyophilized powder

form

powder

form

powder

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

composition

Protein, 20-50% biuret

composition

-

composition

-

composition

-

greener alternative product characteristics

Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

greener alternative product characteristics

Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

greener alternative product characteristics

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

greener alternative product characteristics

Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been used as enzyme for alternative energy. For more information see the Enzymes for Alternative Energy Research.

Application

Pectinesterase is used to hydrolyze methyl esters of pectin to pectate and methanol. It contains pectin methylesterase and pectin pectylhydrolase. Product P5400 is from orange peel and comes as a lyophilized powder. It contains (NH4)2SO4 and sodium chloride. It has been used to study the pectin methylesterase gene[1].
The enzyme from Sigma has been used as a standard during the measurement of pectin methylesterase activity in sea buckthorn juices.[2] It has been used in the hydrolysis of cell wall pectins while studying phyllotaxis in Arabidopsis.[3] It has also been used to catalyze pectin hydrolysis (using an ultrasonic measurement device) for monitoring biological processes.[4]

Biochem/physiol Actions

Pectinesterase catalyzes the hydrolysis of the methyl esters of pectin to yield pectate and methanol.

Unit Definition

One unit will release 1.0 microequivalent of acid from pectin per min at pH 7.5 at 30 °C.

Physical form

Contains (NH4)2SO4 and sodium chloride

Analysis Note

Protein determined by biuret.

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves
  • Specification Sheet

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    Certificates of Analysis (COA)

    Lot/Batch Number
    SLCB9695
    SLBZ2864
    SLBR7758V
    031M7673V
    031M7673

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    Kerstin Müller et al.
    Plant physiology, 161(1), 305-316 (2012-11-07)
    The methylesterification status of cell wall homogalacturonans, mediated through the action of pectin methylesterases (PMEs), influences the biophysical properties of plant cell walls such as elasticity and porosity, important parameters for cell elongation and water uptake. The completion of seed
    Sergio T de Freitas et al.
    The Plant journal : for cell and molecular biology, 71(5), 824-835 (2012-05-09)
    Blossom-end rot (BER) in tomato fruit (Solanum lycopersicum) is believed to be a calcium (Ca²⁺) deficiency disorder, but the mechanisms involved in its development are poorly understood. Our hypothesis is that high expression of pectin methylesterases (PMEs) increases Ca²⁺ bound
    Yuan Zhou et al.
    Phytochemistry, 76, 46-51 (2012-01-11)
    Aluminum (Al) toxicity is one of the most widespread problems for crop production on acid soils, and nitric oxide (NO) is a key signaling molecule involved in the mediation of various biotic and abiotic stresses in plants. Here we found
    Yuri L Dorokhov et al.
    PLoS pathogens, 8(4), e1002640-e1002640 (2012-04-13)
    Many plants release airborne volatile compounds in response to wounding due to pathogenic assault. These compounds serve as plant defenses and are involved in plant signaling. Here, we study the effects of pectin methylesterase (PME)-generated methanol release from wounded plants
    Doungla E Ngouémazong et al.
    Carbohydrate research, 348, 69-76 (2012-01-03)
    The influence of the degree and pattern of methylesterification (DM and PM, respectively) on the stiffness of Ca(2+)-pectin gels is extensively examined, at various Ca(2+) concentrations. Accordingly, a highly methyl-esterified pectin was selectively de-esterified using NaOH, plant or fungal pectin
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