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A1935

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Photobiotin acetate salt

For labeling DNA probes for hybridizations

Synonym(s):

Biotin {3-[3-(4-azido-2-nitroanilino)-N-methylpropylamino]propylamide} acetate salt, N-(4-Azido-2-nitrophenyl)-N′-(3-biotinylaminopropyl)-N′-methyl-1,3-propanediamine acetate salt

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About This Item

Empirical Formula (Hill Notation):
C23H35N9O4S · C2H4O2
CAS Number:
Molecular Weight:
593.70
MDL number:
UNSPSC Code:
12352200
PubChem Substance ID:
NACRES:
NA.52
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grade

Molecular Biology

Quality Level

Assay

≥95%

form

film or powder

solubility

H2O: 10 mg/mL (Stable for at least 5 months if kept frozen and protected from light.)

storage temp.

−20°C

SMILES string

CC(O)=O.CN(CCCNC(=O)CCCC[C@@H]1SCC2NC(=O)NC12)CCCNc3ccc(cc3[N+]([O-])=O)N=[N+]=[N-]

InChI

1S/C23H35N9O4S.C2H4O2/c1-31(12-4-10-25-17-9-8-16(29-30-24)14-19(17)32(35)36)13-5-11-26-21(33)7-3-2-6-20-22-18(15-37-20)27-23(34)28-22;1-2(3)4/h8-9,14,18,20,22,25H,2-7,10-13,15H2,1H3,(H,26,33)(H2,27,28,34);1H3,(H,3,4)/t18-,20-,22-;/m0./s1

InChI key

FFBLNTOMOSLSQM-AYEYRVMASA-N

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1 of 4

This Item
MABS1995MMABS2036MSAB4501927
Gene Information

human ... MT-ND2(4536)

Gene Information

human ... MT-ATP6(4508)

Gene Information

human ... MT-CYB(4519)

Gene Information

human ... MT-ND1(4535)

biological source

mouse

biological source

mouse

biological source

mouse

biological source

rabbit

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

human

clone

9E12-1B3, monoclonal

clone

1G7-1G2, monoclonal

clone

5B3-6E3, monoclonal

clone

polyclonal

antibody form

purified antibody

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

technique(s)

western blot: suitable

technique(s)

western blot: suitable

technique(s)

western blot: suitable

technique(s)

ELISA: 1:40000, immunohistochemistry: 1:50-1:100, western blot: 1:500-1:1000

General description

Photobiotin acetate is made up of biotin, which is bound with the help of a charged linker arm to a photoreactive arylazide group.[1]

Application

Photobiotin acetate salt has been used to label DNA probes to perform DNA−DNA hybridization[2] to determine genomic relatedness.[3][4]

Biochem/physiol Actions

Photobiotin acetate is capable of labeling single stranded DNA and linear or supercoiled double-stranded DNA.[1]
Photobiotin is a photo-activatable analog of biotin used primarily for labeling DNA, RNA and proteins.[5] Probes prepared using photobiotin can detect target DNA as little as 0.5 pg.

Caution

Light sensitive.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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