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About This Item
Linear Formula:
C8H6N3O2Na
CAS Number:
Molecular Weight:
199.14
MDL number:
UNSPSC Code:
12352204
PubChem Substance ID:
NACRES:
NA.83
Product Name
Luminol sodium salt,
Assay
≥98% (titration)
Quality Level
form
powder and chunks
solubility
water: 50 mg/mL, clear to very slightly hazy, colorless to faint red
SMILES string
O=C1NN([Na])C(C2=CC=CC(N)=C21)=O
InChI
1S/C8H7N3O2.Na/c9-5-3-1-2-4-6(5)8(13)11-10-7(4)12;/h1-3H,(H4,9,10,11,12,13);/q;+1/p-1
InChI key
RVJVDCVIJCBUTH-UHFFFAOYSA-M
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Application
Luminol sodium salt has been used:
- as a component of MEBSS buffer in a bioluminescence assay to detect peroxidase activity in whole blood samples
- to study protein interaction in whole-cell lysates by immunoprecipitation
- for in vitro reactive oxygen species (ROS) quantification in CD45+ and CD45− cells
Biochem/physiol Actions
Luminol is a redox-sensitive chemiluminescent compound. It emits blue luminescence when the basic aqueous solution of luminol having oxygen atoms come in contact with oxidizing agents like potassium ferricyanide. It is highly stable and is applicable in various fields such as analytical chemistry, metallurgy, biochemistry, and forensics for detecting reactive species. Luminol has the potential to detect defects in neutrophils ex vivo during oxidative metabolism defects such as chronic granulomatous disease and myeloperoxidase deficiency.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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