biological source
mouse
Quality Level
conjugate
alkaline phosphatase conjugate
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
HIS-1, monoclonal
form
buffered aqueous glycerol solution
technique(s)
western blot: 1:2,000 using lysates of Escherichia coli induced to express a 6xHis tagged protein
isotype
IgG2a
shipped in
wet ice
storage temp.
2-8°C
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General description
The Monoclonal Anti-polyHistidine-Alkaline Phosphatase antibody recognizes native and denatured-reduced forms of synthetic polyhistidine or polyhistidine tagged fusion proteins. The product is reactive with fusion protein expressed by prokaryotic pET, pRSET and pTrc expression vectors.
Specificity
The antibody recognizes synthetic polyHistidine, as well as native or denatured, reduced forms of proteins tagged with 6X histidines, expressed in selected vectors.
Immunogen
recombinant polyHistidine tagged fusion protein.
Application
Antibody is suitable for ELISA, immunoblotting and dot blotting assays.
Physical form
Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl2, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide
Legal Information
This Product is covered by patent DE 19507166 and foreign equivalents exclusively licensed to QIAGEN GmbH, Qiagen Strasse 1, D-40724 Hilden, Germany.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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