C0230

Monoclonal Anti-m/μ Calpain, Small Subunit antibody produced in mouse

~1 mg/mL, clone 28F3, purified immunoglobulin, buffered aqueous glycerol solution

• MDL number: MFCD01864149
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 28F3, monoclonal
• form: buffered aqueous glycerol solution
• mol wt: antigen 28-30 kDa
• species reactivity: bovine, human
• concentration: ~1 mg/mL
• technique(s): immunoprecipitation (IP): suitable; western blot: 1:5,000 using normal human fibroblasts or MDBK cells
• isotype: IgG1
• UniProt accession no.: Q96L46
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... CAPNS1(826), CAPNS2(84290)

Description

General description

Calpain small subunit 1 (Capns1) is a small regulatory subunit associated with the μ-calpain (calpain 1) and m-calpain (calpain 2). The CAPNS1 gene is mapped to human chromosome 19q13.12.

Immunogen

purified calpain from human placenta.

Application

Monoclonal Anti-m/μ Calpain, Small Subunit antibody produced in mouse has been used in immunoprecipitation and immunoblotting.

Biochem/physiol Actions

Calpain small subunit 1 (Capns1) participates in the actin cytoskeleton rearrangement and cell migration events. It is crucial for embryonic development. Capns1 interacts with the Ras GTPase activating protein Src homology 3 domain (SH3) (RasGAP-SH3) domain to regulate cell migration and cell survival. It is essential for calpain activity. Capns1 is associated with the oncogenesis of many tumor types including hepatocellular, colorectal, ovarian, and breast cancer.

Physical form

Solution in 0.02 M sodium phosphate, pH 7.5, and 0.15 M sodium chloride containing 50% glycerol and 3 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable