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Key Documents

C0858

Sigma-Aldrich

Cyclooxygenase 2 human

≥70% (SDS-PAGE), recombinant, expressed in insect cells, aqueous solution, ≥8000 units/mg protein

Synonym(s):

COX-2, Prostaglandin H synthase 2

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About This Item

MDL number:
UNSPSC Code:
12352204
NACRES:
NA.32

recombinant

expressed in insect cells Active recombinant N-terminal His-tagged COX-2 N580A mutant purified from insect cells.
expressed in insect cells

Quality Level

Assay

≥70% (SDS-PAGE)

form

aqueous solution

specific activity

≥8000 units/mg protein

mol wt

dimer subunit mol wt 70-74 kDa

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... PTGS2(5743)

Biochem/physiol Actions

COX-2 catalyzes the conversion of arachidonic acid to prostaglandin H2 (the first step in the biosynthesis of prostaglandins, thromboxanes, and prostacyclins). It is the inducible form of cyclooxygenase, induced by phorbol esters, lipopolysaccharides, and cytokines. COX-2 is upregulated in the neovasculature of malignant tumors. Inhibition of COX-2 with nonsteroidal anti-inflammatory drugs (NSAIDs) is associated with decreased tumor growth.

Other Notes

Tagged with a six-residue histidine sequence near the N-terminus.

Unit Definition

One unit will consume 1.0 nanomole of oxygen per minute at pH 8 at 37 °C.

Physical form

Solution in 80 mM Tris, pH 8.0, with 0.3 mM DDC, 0.01% TWEEN® 20 and 10% glycerol

Substrates

KM for arachidonate = 6.5 μM. Exhibits activity and sensitivity to NSAIDs similar to the non-tagged enzyme.

Legal Information

TWEEN is a registered trademark of Croda International PLC

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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