C0858

Cyclooxygenase 2 human

≥70% (SDS-PAGE), recombinant, expressed in insect cells, aqueous solution, ≥8000 units/mg protein

• Synonym(s): COX-2, Prostaglandin H synthase 2
• MDL number: MFCD00071378
• UNSPSC Code: 12352204
• NACRES: NA.32

Properties

• recombinant: expressed in insect cells Active recombinant N-terminal His-tagged COX-2 N580A mutant purified from insect cells.; expressed in insect cells
• Quality Level: 200
• Assay: ≥70% (SDS-PAGE)
• form: aqueous solution
• specific activity: ≥8000 units/mg protein
• mol wt: dimer subunit mol wt 70-74 kDa
• UniProt accession no.: P35354
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... PTGS2(5743)

Description

Biochem/physiol Actions

COX-2 catalyzes the conversion of arachidonic acid to prostaglandin H₂ (the first step in the biosynthesis of prostaglandins, thromboxanes, and prostacyclins). It is the inducible form of cyclooxygenase, induced by phorbol esters, lipopolysaccharides, and cytokines. COX-2 is upregulated in the neovasculature of malignant tumors. Inhibition of COX-2 with nonsteroidal anti-inflammatory drugs (NSAIDs) is associated with decreased tumor growth.

Other Notes

Tagged with a six-residue histidine sequence near the N-terminus.

Unit Definition

One unit will consume 1.0 nanomole of oxygen per minute at pH 8 at 37 °C.

Physical form

Solution in 80 mM Tris, pH 8.0, with 0.3 mM DDC, 0.01% TWEEN^® 20 and 10% glycerol

Substrates

K_M for arachidonate = 6.5 μM. Exhibits activity and sensitivity to NSAIDs similar to the non-tagged enzyme.

Legal Information

TWEEN is a registered trademark of Croda International PLC

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable