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C3028

Sigma-Aldrich

Colipase from porcine pancreas

essentially salt-free, lyophilized powder

Synonym(s):

Colipase from hog pancreas

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5 G
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5 G
₹54,989.00

About This Item

CAS Number:
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

₹54,989.00


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biological source

Porcine pancreas

Quality Level

Assay

≥95% (Lowry)

form

essentially salt-free, lyophilized powder

concentration

≥500 μ protein/vial

color

white to faint yellow

solubility

H2O: soluble 1.9-2.1 mg/mL, clear, colorless to faintly yellow

suitability

suitable for molecular biology

UniProt accession no.

application(s)

food and beverages

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General description

Research Area: Cell Signaling
Colipase is a member of a group of small cysteine-rich proteins characterized by a limited secondary structure. Its structure is relatively flat, measuring 25×30×35 Å, resembling an arrangement of protruding fingers interconnected at one end by a complex network of five disulfide bridges. The colipase structure comprises two small domains with closely related topology.[1]
The gene colipase (CLPS) encodes a lipolytic enzyme that is majorly expressed in the exocrine pancreas. This protein is also found in the stomach and intestine. Its structure includes 17 amino acid signal peptides and a five amino acid pro-piece at the N-terminus.

Application

Colipase from porcine pancreas has been used:

  • in the enzyme solution for lipase assay
  • in an enzyme mixture to simulate gastrointestinal digestion
  • as one of the apparent critical factors to study its effect on lycopene in vitro accessibility

Biochem/physiol Actions

Colipase is involved in lipid metabolism and apoptosis signaling. It is crucial for fat digestion. It is a pancreatic protein that prevents the denaturation of lipase and enables its attachment to the lipid-water interphase of the droplet. Colipase makes lipase accessible to the inner core of triacylglycerol. Defective colipase secretion induced by an insufficient exocrine pancreatic function minimizes the luminal hydrolysis of dietary fat. Lack of congenital lipase or colipase causes pancreatic fat malabsorption.
Colipase, a small protein cofactor, is essential for efficient dietary lipid hydrolysis by pancreatic lipase. It attaches to the non-catalytic C-terminal domain of the lipase, stabilizing an active conformation and significantly enhancing the overall hydrophobic binding site.[1] This pancreatic exocrine protein aids in the adsorption of pancreatic triglyceride lipase (PTL) to the substrate lipid-water interface.[2]

Features and Benefits

  • Overcomes the inhibition of lipase by bile salts.
  • Activity can be demonstrated turbidimetrically in an emulsion of triolein and sodium deoxycholate together with porcine pancreatic lipase.

Preparation Note

Dissolves in water to form a clear, colorless to faint yellow colored solution at 1.9-2.1 mg/mL concentration.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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    A J Bridges et al.
    Journal of medicinal chemistry, 39(1), 267-276 (1996-01-05)
    4-(3-Bromoanilino)-6,7-dimethoxyquinazoline (32, PD 153035) is a very potent inhibitor (IC50 0.025 nM) of the tyrosine kinase activity of the epidermal growth factor receptor (EGFR), binding competitively at the ATP site. Structure-activity relationships for close analogues of 32 are very steep.
    D W Fry et al.
    Science (New York, N.Y.), 265(5175), 1093-1095 (1994-08-19)
    A small molecule called PD 153035 inhibited the epidermal growth factor (EGF) receptor tyrosine kinase with a 5-pM inhibition constant. The inhibitor was specific for the EGF receptor tyrosine kinase and inhibited other purified tyrosine kinases only at micromolar or
    Albrecht Stenzinger et al.
    Cells, tissues, organs, 184(2), 76-87 (2007-03-16)
    The novel protein PTPIP51 (protein tyrosine phosphatase-interacting protein 51), which has been found to interact with protein tyrosine phosphatases of the PTP1B/TcPTP subfamily, is expressed in all suprabasal layers of human epidermis. Hence, a human keratinocyte cell line (HaCaT) grown

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