biological source
rat colon
packaging
tube of 5 μg 90062901-DNA-5UG
pkg of vial of cells 90062901-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Related Categories
Cell Line Origin
Rat colonic carcinoma
Cell Line Description
This cell line has properties essential for study of metastasis of colon carcinomas; a reproducible pattern of metastatic behaviour and ability to be propagated both in vitro and in vivo to facilitate identification of biochemical correlates of metastasis.
Application
Metastasis studies
Culture Medium
DMEM + F10 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) or 2% Rat Serum.
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:5 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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