D4764

Deoxyribonucleic acid from calf thymus

Genomic, unsheared

• Synonym(s): double-stranded template DNA, Calf Thymus DNA, DNA from calf thymus, Thymonucleic acid
• CAS Number: 91080-16-9
• EC Number: 293-507-3
• MDL number: MFCD00151698
• UNSPSC Code: 41106310
• eCl@ss: 32160414
• NACRES: NA.52

Properties

• grade: Molecular Biology
• Quality Level: 200
• form: lyophilized powder
• mol wt: >20 kb
• storage temp.: 2-8°C

Description

General description

High quality double-stranded template DNA isolated from the thymus of male and female calves.

Specificity

42% GC; T_m = 87 °C.
% GC is the percentage of G/C base pairs. T_m is the temperature at the midpoint of the thermal denaturation profile.

Application

Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations.

Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations. It was used as a standard for DNA quantification by fluorescent assay. It was used as reference to determine the DNA content in cartilage tissue digest samples.

Features and Benefits

• No nuclease activity detected after 16 h at 37 °C incubation
• High quality template DNA

Preparation Note

Purified by anion exchange chromatography, dialyzed against a solution of 1 mM sodium chloride, 1 mM EDTA and 1 mM Trizma HCl, pH 7.5 and lyophilized at a concentration of ~2 A₂₆₀ units per mL. ~20 A₂₆₀ units per mg DNA.

Reconstitution

Dissolve the DNA gently rocking overnight to a final concentration of 1mg/ml in molecular biology grade water.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves