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F1757

Sigma-Aldrich

Fumarase from porcine heart

ammonium sulfate suspension, ≥300 units/mg protein (biuret)

Synonym(s):

L-Malate hydrolyase, Fumarate hydratase

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250 μG
405,00 $

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250 μG
405,00 $

About This Item

CAS Number:
EC Number:
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

405,00 $


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form

ammonium sulfate suspension

Quality Level

specific activity

≥300 units/mg protein (biuret)

foreign activity

malic dehydrogenase ≤0.1%

storage temp.

2-8°C

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This Item
PHR1673I701690656
grade

analytical standard

grade

certified reference material, pharmaceutical secondary standard

grade

-

grade

analytical standard

application(s)

food and beverages

application(s)

pharmaceutical (small molecule)

application(s)

-

application(s)

food and beverages

format

single component solution

format

neat

format

-

format

single component solution

shelf life

limited shelf life, expiry date on the label

shelf life

-

shelf life

-

shelf life

limited shelf life, expiry date on the label

concentration

0.1 mg/mL in isooctane

concentration

-

concentration

-

concentration

0.1 mg/mL in isooctane

storage temp.

−20°C

storage temp.

2-30°C

storage temp.

2-8°C

storage temp.

−20°C

General description

Fumarase (FH in human) is a well-known tricarboxylic-acid-cycle enzyme found in both the cytoplasm and mitochondria of all eukaryotes.[1]
Fumarase is mapped to human chromosome 1q43.[2] Fumarase comprises of three domains D1, D2 and D3 and belongs to class II fumarase family.[3]

Application

Fumarase from porcine heart has been used:
  • as a positive control in isocitrate dehydrogenase and α-ketoglutarate dehydrogenase assay[4]
  • for immobilized enzyme based stability assay[5]
  • in fumarate hydratase assays in human lung carcinoma cells (A549)[6]

Fumarase is used as a protein calibration standard in the purification of intact DNA polymerase a/primase from mouse cells.[7]

Biochem/physiol Actions

Fumarase catalyzes the reversible hydration of fumarate to malate. In its mitochondrial form, fumarate is involved in the Krebs Cycle, while the cytosolic form is involved in amino acid metabolism.
Fumarase is essential for regulation of cellular metabolism.[8] Fumarase elicits protective function from double-strand breaks (DSBs) induced by infrared radiation.[9] Loss of function mutations in the fumarase gene is implicated in hereditary leiomyomatosis and renal cell cancer (HLRCC)-syndrome.[2][10]

Unit Definition

One unit will convert 1.0 μmole of L-malate to fumarate per min at pH 7.6 at 25 °C.

Physical form

Suspension in 3.2 M (NH4)2SO4, 0.05 M KH2PO4, pH 7.5, 0.014 M 2-mercaptoethanol

Preparation Note

Isolated by the method of Beeckmans, S., and Kanarek, L., Eur. J. Biochem., 78, 437 (1977). Affinity purified.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Skin Sens. 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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