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Key Documents

F2637

Sigma-Aldrich

Ficoll® 400

BioXtra, lyophilized powder

Synonym(s):

Polysucrose 400, Ficoll 400

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About This Item

CAS Number:
MDL number:
UNSPSC Code:
12352200
NACRES:
NA.52

biological source

non-animal source

Quality Level

grade

Molecular Biology

product line

BioXtra

form

lyophilized powder

foreign activity

DNase (Exonuclease), NICKase (Endonuclease), RNase and Protease, none detected

storage temp.

room temp

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General description

Ficoll® 400 is a non-ionic synthetic polymer of sucrose used for cell separation and organ isolation.

Application

Suitable for
  • hybridization experiments to reduce non-specific binding of labeled probes to the membrane
  • preparation of DNA loading buffer (15% Ficoll®, 0.25% bromophenol blue and 0.25% xylene cyanol FF
  • separation of cells with similar density but different size (PBMC from human peripheral blood, bone marrow and umbilical cord blood)
  • producing density gradients to separate subcellular components
  • vitrification of cell and tissue samples.
  • to compare the effect of different perfusates on Hypoxic pulmonary vasoconstriction (HPV).
Used for cell separation and organelle isolation.

Features and Benefits

  • free from DNases, RNases, phosphatases and proteases
  • preserves functional and morphological integrity of the separated cells

Other Notes

A nonionic synthetic polymer of sucrose.

Legal Information

Ficoll is a registered trademark of Cytiva

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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