F2637

Ficoll^® 400

BioXtra, lyophilized powder

• Synonym(s): Polysucrose 400, Ficoll 400
• CAS Number: 26873-85-8
• MDL number: MFCD00081599
• UNSPSC Code: 12352200
• NACRES: NA.52

Properties

• biological source: non-animal source
• Quality Level: 200
• grade: Molecular Biology
• product line: BioXtra
• form: lyophilized powder
• foreign activity: DNase (Exonuclease), NICKase (Endonuclease), RNase and Protease, none detected
• storage temp.: room temp

Description

General description

Ficoll^® 400 is a non-ionic synthetic polymer of sucrose used for cell separation and organ isolation.

Application

Suitable for

• hybridization experiments to reduce non-specific binding of labeled probes to the membrane
• preparation of DNA loading buffer (15% Ficoll^®, 0.25% bromophenol blue and 0.25% xylene cyanol FF
• separation of cells with similar density but different size (PBMC from human peripheral blood, bone marrow and umbilical cord blood)
• producing density gradients to separate subcellular components
• vitrification of cell and tissue samples.
• to compare the effect of different perfusates on Hypoxic pulmonary vasoconstriction (HPV).

Used for cell separation and organelle isolation.

Features and Benefits

• free from DNases, RNases, phosphatases and proteases
• preserves functional and morphological integrity of the separated cells

Other Notes

A nonionic synthetic polymer of sucrose.

Legal Information

Ficoll is a registered trademark of Cytiva

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves