F6057

Fluoroshield^™ with DAPI

liquid

• UNSPSC Code: 12171500
• NACRES: NA.47

Properties

• Product Name: Fluoroshield^™ with DAPI, liquid
• description: histology mounting medium
• Quality Level: 200
• form: liquid
• composition: Sodium Azide, ≤1% (also contains other ingredients); Tris-HCl
• technique(s): immunofluorescence: suitable
• pH range: 7.9-8.3
• solubility: water: soluble
• fluorescence: λ_ex 360 nm; λ_em 460 nm
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: 2-8°C

Description

General description

Fluoroshield with DAPI is an aqueous mounting medium fortified with 4′,6-diamidino-2-phenylindole (DAPI), a nuclear counterstain used as a blue-fluorescent DNA probe. It is an anti-fading agent employed for preserving the fluorescence of tissue and cell smears by preventing rapid photobleaching and minimizing the blinking-mediated phenomena.(1) For use in in situ hybridization techniques or other methods where fluorescence of DNA staining is required. DAPI excites at 360 nm and emits at 460 nm, producing a blue fluorescence. RNA is also stained with DAPI.

Application

Fluoroshield^™ with DAPI has been used:

• as a counterstain in immunocytochemistry to label nuclei of neural progenitor cells
• as a histology mounting medium for cardiac tissues sections for immunofluorescence studies
• as mounting solution for immunostained heart sections for nuclei detection

Legal Information

FluoroShield is a trademark of ImmunoBioScience Corp.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable