G0657

GROα human

≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonym(s): MGSA, Melanoma Growth Stimulating Activity
• EC Number: 200-835-2
• MDL number: MFCD00677959
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• biological source: human
• Quality Level: 100
• recombinant: expressed in E. coli
• Assay: ≥97% (SDS-PAGE)
• form: lyophilized powder
• potency: 1.0-4.0 ng/mL
• mol wt: ~7.9 kDa
• packaging: pkg of 10 μg
• storage condition: avoid repeated freeze/thaw cycles
• technique(s): cell culture | mammalian: suitable

Description

General description

GROα, also called CXCL1 (chemokine (C-X-C motif) ligand 1), is a member of the small soluble chemokine protein family. It belongs to the C-X-C subfamily, which is comprised of 17 members. It contains a conserved glutamic acid-leucine-arginine (ELR) domain. This protein is expressed on stromal cells such as, endothelial cells, fibroblasts and blood-circulating cells.[1]

Biochem/physiol Actions

GROα, also called CXCL1 (chemokine (C-X-C motif) ligand 1), plays an essential role in neutrophil recruitment, and the initiation of angiogenesis. This gene is up-regulated in multiple cancer types such as, gastric cancer, renal cell carcinoma, prostate cancer and melanoma. The expression of this protein is also elevated in HER2 positive metastatic breast cancer. Its expression is increased in fibroblasts, which might be involved in breast tumor progression, and its stromal expression in breast cancer is linked to poor prognosis.[1] This gene is also up-regulated in the aggressive phenotype of bladder cancer.[2] Polymorphism rs4074 A in this gene is also associated with susceptibility to alcoholic cirrhosis.[3]

Physical form

Lyophilized from a 0.2 μm filtered solution in 25% acetonitrile and 0.1% trifluoroacetic acid containing 50 μg bovine serum albumin per 1 μg of cytokine.

Analysis Note

The biological activity is measured by its ability to induce chemotaxis of mouse BaF/3 cells transfected with hCXCR2.

Safety Information

• Pictograms: GHS02,GHS07
• Signal Word: Danger
• Hazard Statements: H225,H302 + H312 + H332,H319
• Precautionary Statements: P210 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P312 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 2
• Storage Class Code: 3 - Flammable liquids
• WGK: WGK 2
• Flash Point(F): 35.6 °F - (Acetonitrile)
• Flash Point(C): 2.0 °C - (Acetonitrile)
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves