Name: β-Glucuronidase from <I>Escherichia coli</I>
Brand: SIGMA
Price: 723 MYR

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4-Pyridinecarbonitrile

recombinant

expressed in E. coli

Quality Level

200

form

aqueous glycerol solution

specific activity

>20,000,000 units/g protein

mol wt

74 kDa

concentration

>1 mg/mL

shipped in

wet ice

storage temp.

−20°C

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Show Differences

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This Item	G8162	G8885	G0251
G0799 β-Glucuronidase from Escherichia coli	G8162 β-Glucuronidase from Escherichia coli	G8885 β-Glucuronidase from Helix pomatia	G0251 β-Glucuronidase from bovine liver
specific activity >20,000,000 units/g protein	specific activity ≥5,000,000 units/g protein, pH 6.8 (biuret)	specific activity ≥90,000 units/mL	specific activity ≥1,000,000 units/g solid
concentration >1 mg/mL	concentration -	concentration -	concentration -
recombinant expressed in E. coli	recombinant -	recombinant -	recombinant -
form aqueous glycerol solution	form aqueous glycerol solution	form aqueous solution	form solid
shipped in wet ice	shipped in wet ice	shipped in wet ice	shipped in -
mol wt 74 kDa	mol wt 69-71 kDa	mol wt -	mol wt -

General description

β-Glucuronidase is a tetramer belonging to the glycosidase family with a molecular weight of 29 kDa. It is composed of 74 kDa identical monomers. The pI and optimal pH of the enzyme is 4.8 and 6-7 respectively.[1]

Application

β-Glucuronidase was used in the study of Cer-β-glucuronide synthesis and evaluation of β-glucuronidase(s) from Escherichia coli or intestinal segments to release the head group.[2]

New Technical Article Comparing Performance of Different Enzymes
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about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine

Effective in the hydrolysis of steroid glucuronides.

Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis

Unit Definition

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at the pH 6.8 (30 min assay).

Physical form

β-glucuronidase is used as a reporter gene in GUS assays to monitor gene expression.

Highly purified solution in 50% glycerol

Pictograms

GHS08

Signal Word

Danger

Hazard Statements

H317,H334

Precautionary Statements

P261 - P280 - P342 + P311

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number

SLBB6139V

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β-Glucuronidase from Escherichia coli

>20,000,000 units/g protein, recombinant, expressed in E. coli, aqueous glycerol solution

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