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H7016

Sigma-Aldrich

Hirudin

from leeches, ≥1,500 ATU/mg protein, lyophilized powder

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20 μG
MYR 741.00
50 μG
MYR 1,323.00

About This Item

CAS Number:
8001-27-2
EC Number:
232-279-1
MDL number:
MFCD00081692
UNSPSC Code:
12352202

MYR 741.00

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Product Name

Hirudin from leeches, lyophilized powder, ≥1,500 ATU/mg protein (ATU = antithrombin units)

biological source

animal (Hirudo verbana)

Quality Level

100
200

form

lyophilized powder

specific activity

≥1,500 ATU/mg protein (ATU = antithrombin units)

mol wt

7 kDa

solubility

pyridine: soluble
water: soluble

storage temp.

2-8°C

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Ascentis® Express 90 Å AQ-C18, 2.7 μm HPLC Guard Cartridge pack of 3 ea, cartridge L × I.D. 0.5 cm × 3 mm

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577161-U

Ascentis® Express 90 Å AQ-C18, 2.0 μm HPLC Guard Cartridge

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577340-U

Ascentis® Express 90 Å AQ-C18, 2.7 μm HPLC Guard Cartridge

separation technique

reversed phase

separation technique

reversed phase

separation technique

reversed phase

separation technique

reversed phase

matrix active group

AQ-C18, polar modified bonding phase

matrix active group

AQ-C18, polar modified bonding phase

matrix active group

AQ-C18, polar modified bonding phase

matrix active group

AQ-C18, polar modified bonding phase

particle size

2.7 μm

particle size

2.7 μm

particle size

2 μm

particle size

2.7 μm

product line

Ascentis®

product line

Ascentis®

product line

Ascentis®

product line

Ascentis®

pore size

90 Å pore size

pore size

90 Å pore size

pore size

90 Å pore size

pore size

90 Å pore size

agency

suitable for USP L1

agency

suitable for USP L1

agency

suitable for USP L1

agency

suitable for USP L1

General description

Hirudin is a 7 kDa acidic protein containing 65 amino acid residues that has an isoelectric point of 3.5-4.0. It is not glycosylated and lacks tryptophan, arginine and methionine residues. It is chemically stable between pH 2-12 and at temperatures up to 80°C.

Biochem/physiol Actions

The anticoagulant, hirudin, is the most potent natural inhibitor of both soluble and clot-bound thrombin. It binds thrombin with high affinity, covering more than 20% of its surface area and occluding both the active site and exosite I (fibrinogen and PAR recognition site). Hirudin blocks thrombus growth and platelet activation. It is not metabolized in the bloodstream of humans and is eliminated unchanged via kidney filtration.
The anticoagulant, hirudin, is the most potent natural inhibitor of both soluble and clot-bound thrombin. It forms a high-affinity 1:1 complex with thrombin, occluding both the proteolytic site and exosite I (fibrinogen and PAR recognition site). Hirudin blocks thrombus growth and platelet activation, and has been suggested as the clinically preferred anticoagulant (over heparin and citrate) for its specific mode of action and absence of side effects. It is not metabolized in the bloodstream of humans and is eliminated unchanged via kidney filtration.

Unit Definition

One unit (ATU) will neutralize one NIH unit of thrombin at 37 °C, based on direct comparison to an NIH thrombin reference standard.

Analysis Note

Protein determined by Lowry.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves
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