I1643

Pyrophosphatase, Inorganic from baker′s yeast (S. cerevisiae)

powder, ≥500 units/mg protein (E1%/280)

• Synonym(s): Inorganic Pyrophosphatase, Pyrophosphate phosphohydrolase
• CAS Number: 9024-82-2
• EC Number: 3.6.1.1 (BRENDA, IUBMB)
• EC Number: 232-784-7
• MDL number: MFCD00131379
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• form: powder
• Quality Level: 300
• specific activity: ≥500 units/mg protein (E1%/280)
• mol wt: 71 kDa
• storage temp.: −20°C

Description

General description

Molecular Weight: 71 kDa (homodimer consisting of two equal subunits of molecular weight 32-35 kDa)
Extinction Coefficient: E^1% = 14.5 (280 nm)
pI: 4.75
This ubiquitous enzyme serves to drive metabolic reactions that produce pyrophosphate, since these reactions typically have equilibrium constants near unity. The catalytic mechanism has been described in the literature. Inorganic pyrophosphatase is a metalloprotease that requires Mg²⁺ for maximal activity. Although the hydrolysis of inorganic pyrophosphate is specific in the presence of Mg²⁺, both ADP and ATP can be hydrolyzed if zinc is present.

Application

Inorganic pyrophosphatase (PPase) is a ubiquitous enzyme catalyzing the reaction PP_i + H₂O → 2P_i.
It plays an important role in protein, RNA, and DNA synthesis.

Pyrophosphatase has been used:

• as a component of the reaction buffer used in aminoacylation assay to quantify incorporation of [3H]L-methionine into tRNA.[1]
• as a component of PCR mixture.[2]

Pyrophosphatase has been used:
as a component of the reaction buffer used in aminoacylation assay to quantify incorporation of [3H]L-methionine into tRNA.
as a component of PCR mixture

Unit Definition

One unit will liberate 1.0 μmole of inorganic orthophosphate per min at pH 7.2 at 25 °C.

Physical form

Lyophilized powder containing 80-90% buffer salts

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves