I9271

5-(Iodoacetamido)fluorescein

≥90% purity (HPLC), powder

• Synonym(s): 5-IAF
• Empirical Formula (Hill Notation): C₂₂H₁₄INO₆
• CAS Number: 63368-54-7
• Molecular Weight: 515.25
• Beilstein: 6543244
• UNSPSC Code: 12171500
• PubChem Substance ID: 329815467
• NACRES: NA.47

Properties

• Product Name: 5-(Iodoacetamido)fluorescein, ≥90% (HPLC)
• Quality Level: 200
• Assay: ≥90% (HPLC)
• form: powder
• technique(s): titration: suitable
• color: yellow to orange
• solubility: DMSO: 5 mg/mL
• fluorescence: λ_ex 492 nm; λ_em 515 nm (Mercaptoethanol adduct); λ_ex 493 nm; λ_em 522 nm (pH 9.2)
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: −20°C
• SMILES string: Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(NC(=O)CI)ccc45)c1
• InChI: 1S/C22H14INO6/c23-10-20(27)24-11-1-4-15-14(7-11)21(28)30-22(15)16-5-2-12(25)8-18(16)29-19-9-13(26)3-6-17(19)22/h1-9,25-26H,10H2,(H,24,27)
• InChI key: UATCLPJEZJKNHE-UHFFFAOYSA-N

Description

General description

5-(Iodoacetamido)fluorescein is a thiol reactive fluorescein derivative.

Application

5-(Iodoacetamido)fluorescein is used for the synthesis of fluorescently labeled organelles (nuclei and nuclear matrices), proteins and peptides that include enzymes.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves