Name: Lucifer Yellow CH dipotassium salt
Brand: SIGMA

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About This Item

Empirical Formula (Hill Notation):

C₁₃H₉K₂N₅O₉S₂

CAS Number:

71206-95-6

Molecular Weight:

521.57

Beilstein:

5224942

MDL number:

MFCD00013445

UNSPSC Code:

12171500

PubChem Substance ID:

24896249

NACRES:

NA.77

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form

powder

Quality Level

100

color

orange

fluorescence

λ_ex 428 nm; λ_em 540 nm

storage temp.

room temp

SMILES string

[K+].[K+].NNC(=O)NN1C(=O)c2cc(cc3c(N)c(cc(C1=O)c23)S([O-])(=O)=O)S([O-])(=O)=O

InChI

1S/C13H11N5O9S2.2K/c14-10-5-1-4(28(22,23)24)2-6-9(5)7(3-8(10)29(25,26)27)12(20)18(11(6)19)17-13(21)16-15;;/h1-3H,14-15H2,(H2,16,17,21)(H,22,23,24)(H,25,26,27);;/q;2*+1/p-2

InChI key

HCWYSCVNSCIZCN-UHFFFAOYSA-L

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General description

Lucifer yellow CH is a fixable dye with an excitation and emission spectrum of 430 nm and 540 nm, respectively.LuciferYellow CH (LYCH) is a small, hydrophilic molecule, and highly fluorescent dye that is used for intracellular staining for the studies conducted on various types of cells. It acts as a marker for the detection of well-established tight junctions in smooth muscles, glia, and interstitial cells of Cajal (ICC).

Application

Lucifer yellow CH dipotassium salt has been used:

to visualize cell morphology
to assess intercellular communication activity through gap junction by scrape-loading assay
as a component of the intracellular solution, which filled the dendrites for the visualization of neuronal morphology
in the intracellular solution, to intracellularly label recorded neurons

Features and Benefits

Highly fluorescent dye, useful in marking nerve cells.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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