M1891

Myelin Basic Protein bovine

≥90% (SDS-PAGE), lyophilized powder, suitable for activity assay

• Synonym(s): MBP
• MDL number: MFCD00131640
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• biological source: bovine
• Quality Level: 200
• Assay: ≥90% (SDS-PAGE)
• form: lyophilized powder
• mol wt: 18.4 kDa
• storage condition: (Keep container tightly closed in a dry and well-ventilated place)
• technique(s): activity assay: suitable
• solubility: water: 1 mg/mL, clear, colorless to faintly yellow
• UniProt accession no.: P02687
• storage temp.: −20°C

Description

General description

Research Area: Cell Signaling

Myelin basic protein (MBP), a single-chain polypeptide consisting of 170 amino acids[1] with a methyl-arginine, is a peripheral membrane protein.[2] This crucial structural component of the myelin sheath is the second-most abundant protein in myelin representing about 30% of the total myelin protein.[3]

Application

Myelin Basic Protein (MBP) bovine has been used:

• in in vitro methylation reaction[4][5]
• as a substrate in in vitro immunoprecipitation-kinase assay to measure Cds1 (the S-phase-specific checkpoint effector kinase) activity[6]
• to prepare phosphorylated MBP, which is used as a substrate in phosphatase assays[7]

Used as a kinase substrate.

Biochem/physiol Actions

Mutation of the myelin basic protein gene induces dysmyelination. It is used to induce experimental allergic encephalomyelitis.

Myelin basic protein (MBP) plays a vital role in the formation of the myelin sheath and is associated with various neurological disorders. MBP functions as an antigenic target in the autoimmune disease experimental allergic encephalomyelitis (EAE). MBP plays an essential role in cytoskeletal turnover and membrane extension. Additionally, it is also involved in Fyn-mediated signaling pathways, sequestration of phosphoinositide, and regulation of calcium homoeostasis.[8] MBP functions as an in vitro substrate for mitogen-activated protein kinase (MAP) kinase and aids in the purification and functional studies of enzymes.[9]MBP serves as a potential neuroreceptor. It can stimulate the proliferation of lymphocytes in vitro. MBP plays a key role as a substrate in the mitogen-activated protein (MAP) kinase activation pathway.[10] Based on lymphocyte stimulation, MBP can be used to detect cancer. It is used in several immunological studies. MBP also contributes to the preservation of the lipid: protein organization within myelin.[2]

Quality

May contain traces of urea-glycine buffer salts.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves