S0191

Sonic Hedgehog Peptide from mouse

≥97% (SDS-PAGE), recombinant, expressed in E. coli, Fused to a histidine tag at the C-terminus, lyophilized powder

• Synonym(s): Shh
• MDL number: MFCD03792594
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• biological source: mouse
• Quality Level: 200
• recombinant: expressed in E. coli
• Assay: ≥97% (SDS-PAGE)
• form: lyophilized powder
• potency: 0.4-3.0 μg/mL ED₅₀
• mol wt: predicted mol wt 20 kDa
• packaging: pkg of 25 μg
• storage condition: avoid repeated freeze/thaw cycles
• impurities: <1 EU/μg
• UniProt accession no.: Q62226
• storage temp.: −20°C
• Gene Information: mouse ... Shh(20423)

Description

General description

Sonic hedgehog (Shh) is a morphogenic glycoprotein. The gene encoding it is localized on mouse chromosome 5.

Application

Sonic hedgehog peptide from mouse has been used for neural differentiation.

Biochem/physiol Actions

Cell signaling peptide involved in embryonic morphogenic development of several tissues including Hensen′s node, the notochord, and the neural tube.

Involved in embryonic morphogenic development of several tissues including Hensen′s node, the notochord, and the neural tube.

Sonic hedgehog (Shh) has a role in fracture healing and regeneration. The protein associates with its receptor Patched (Ptc-1), de-represses Smoothened (Smo) and activates the Gli family of transcription factors. Mutations in the Shh pathway have been linked to developmental diseases and cancer.

Other Notes

N-terminal peptide encoding amino acid residues 25-198.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline, pH 7.4, containing 5% trehalose and 50 μg of bovine serum albumin per 1 μg of cytokine.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable