S4942

SYPRO^® Ruby Protein Gel Stain

• Synonym(s): SYPRO^® dye, protein gel stain
• UNSPSC Code: 41105322
• NACRES: NA.32

Properties

• shelf life: ≥6 mo. (when stored at room temperature and protected from light)
• Quality Level: 100
• technique(s): protein staining: suitable
• fluorescence: λ_ex 280,450 nm; λ_em 610 nm

Description

General description

SYPRO Ruby protein gel stain is a ready-to-use, ultrasensitive, luminescent stain for the detection of proteins separated by polyacrylamide gel electrophoresis (PAGE). This stain, designed especially for use in 2-D PAGE, has proven to be an excellent choice for 1-D PAGE and isoelectric focusing (IEF) gels as well. SYPRO Ruby protein gel stain attains sensitivity comparable to many silver stain techniques. However, unlike silver staining, the SYPRO Ruby gel stain:

• uses a simple staining protocol, with no possibility of overstaining
• delivers a linear quantitation range of over three orders of magnitude
• shows less protein-to-protein variability
• stains glycoproteins, lipoproteins, calcium-binding proteins, fibrillar proteins, and other difficult-to-stain proteins
• will not stain extraneous nucleic acids
• does not interfere with subsequent analysis of proteins by Edman-based sequencing or mass spectrometry

Application

SYPRO ruby protein gel stain has been used for staining of the proteins after sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE).

Caution

Protect from light.

Legal Information

SYPRO is a registered trademark of Life Technologies

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves