S7776
Starch Azure
Insoluble corn starch covalently linked with Remazol Brilliant Blue R
Synonym(s):
Starch–Remazol brilliant blue R, RBB-Starch, Remazol brilliant blue R dyed starch, Starch Azure
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About This Item
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Quality Level
form
powder
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General description
Starch is an insoluble starch substrate specific for α-amylase and not for other exo enzymes. α-amylase catalysis of starch azure solubilization is measured at 595 nm.
Application
Starch Azure has been used as a substrate
- in the screening of α-amylase activity in decontaminated malted flour at varying pH and temperature
- for monitoring the inhibitory activity of Physalis peruviana extracts
- for thermostable α-amylase extracted from deep sea and acidic soil
Substrates
Substrate for colorimetric determination of α-amylase.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Richardson TH, et al.
The Journal of Biological Chemistry, 277(29), 26501-26507 (2002)
The use of starch azure for measurement of alpha-amylase activity
Lehoczki G, et al.
Carbohydrate Polymers, 183, 263-266 (2018)
Inhibitory effects of an extract of fruits of Physalis peruviana on some intestinal carbohydrases
Rey D, et al.
Revista Colombiana de Ciencias Quimico-Farmaceuticas, 44(1), 72-89 (2015)
Modeling cereal starch hydrolysis during simultaneous saccharification and lactic acid fermentation; case of a sorghum-based fermented beverage, gowe
Mestres C, et al.
Food Research International, 100, 102-111 (2017)
Gábor Lehoczki et al.
Carbohydrate polymers, 183, 263-266 (2018-01-22)
Despite being widely used, there is no standard protocol for α-amylase activity measurement with starch azure substrate. Boiling pre-treatment of starch azure suspension increased the reaction rate of hydrolysis catalysed by human salivary α-amylase (HSA) or porcine pancreatic α-amylase (PPA)
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