SRE0053

Lysostaphin from Staphylococcus staphylolyticus

Suitable for manufacturing of diagnostic kits and reagents., lyophilized powder

• Synonym(s): Glycyl-glycine Endopeptidase
• CAS Number: 9011-93-2
• EC Number: 3.4.24.75 (BRENDA, IUBMB)
• MDL number: MFCD00131556
• UNSPSC Code: 12352204
• NACRES: NA.32

Properties

• biological source: bacterial (Staphylococcus sp.)
• Quality Level: 400
• form: lyophilized powder
• specific activity: ≥500 units/mg protein
• mol wt: 26,926 Da
• composition: protein, 50-70% biuret
• packaging: pkg of (package size sold on basis of mg protein)
• concentration: 50—70% protein
• suitability: suitable for microbiological tests
• application(s): diagnostic assay manufacturing
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Lysostaphin is a glycylglycine endopeptidase. It is an important antibacterial enzyme.

Biochem/physiol Actions

Lysostaphin has the ability to cleave both viable and heat-killed S. aureus cells. It is one of the major antibacterial agents for the staphylococci. Lysostaphin cleaves the cross-linking pentaglycine bridges in the cell walls of staphylococci. Thus it is considered as an antistaphylococcal therapeutic agent.

Lysostaphin is a zinc endopeptidase with a molecular weight of approximately 25 kDa. Because lysostaphin cleaves the polyglycine cross-links in the peptidoglycan layer of the cell wall of Staphylococcus species it has been found useful for cell lysis and also as a potential anti-microbial therapeutic.
pH Optimum for activity: ~7.5

Unit Definition

One unit will reduce the turbidity (A₆₂₀) of a suspension of Staphylococcus aureus cells from 0.250 to 0.125 in 10 min at pH7.5 at 37°C in a 6.0ml reaction mixture.

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable