SRP0356

GSK3β active human

recombinant, expressed in baculovirus infected Sf9 cells, ≥80% (SDS-PAGE)

• Synonym(s): glycogen synthase kinase 3 beta
• CAS Number: 443900-95-6
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in baculovirus infected Sf9 cells
• Assay: ≥80% (SDS-PAGE)
• form: aqueous solution
• specific activity: ≥500 pmol/min-μg
• mol wt: 73 kDa
• packaging: pkg of 10 μg
• technique(s): activity assay: suitable; inhibition assay: suitable
• NCBI accession no.: NM_002093
• UniProt accession no.: P49841
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... GSK3B(2932)

Description

General description

Glycogen Synthase Kinase 3β (GSK3β) is one of the two isoforms of GSK3 enzyme, both of which are encoded by different genes. This protein is predominantly cytoplasmic, but is also found to be localized to nucleus and mitochondria. It is a protein-serine kinase. This protein contains a protein kinase domain in its core, and is composed of 482 amino acids with a molecular weight of 46,712Da. It shows a wide range of tissue expression with the highest expression in brain.

Human GSK3beta GenBank Accession No. NM_002093), full length with N-terminal GST-tag, MW=73 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Biochem/physiol Actions

Glycogen Synthase Kinase 3β (GSK3β) is responsible for rapid cyclin D1 turnover by phosphorylating it on Thr-286. It functions as a negative regulator of Wnt signaling during embryonic development and cell proliferation in adult tissues. This protein phosphorylates and regulates multiple proteins such as glycogen synthase, insulin receptor substrate-1, axin and APC (adenomatous polyposis coli). This protein is also shown to interact with multiple protein which are linked with Alzheimer′s disease (AD).

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable