T8657

Trichloroacetic acid

suitable for electrophoresis, suitable for fixing solution (for IEF and PAGE gels), ≥99%

• Synonym(s): TCA
• Linear Formula: Cl₃CCOOH
• CAS Number: 76-03-9
• Molecular Weight: 163.39
• Beilstein: 970119
• EC Number: 200-927-2
• MDL number: MFCD00004177
• UNSPSC Code: 41105319
• PubChem Substance ID: 24900550
• NACRES: NB.22

Properties

• grade: electrophoresis grade
• Quality Level: 200
• vapor density: <1 (vs air)
• vapor pressure: 1 mmHg ( 51 °C)
• Assay: ≥99%
• form: powder or flakes (or crystals)
• technique(s): electrophoresis: suitable
• turbidity: ≤3 NTU (1 g/ml)
• refractive index: n20/D 1.62 (lit.)
• bp: 196 °C (lit.)

Description

General description

Trichloroacetic acid (TCA) is produced during the chlorination of water comprising natural organic material.[1] It is a novel reagent used in clinical laboratories for deproteinization of body fluids.[2] In addition, TCA is also used in the treatment of genital warts[3] and acne scars.[4]

Application

Traditionally used to precipitate protein. Has been used to determine protein concentration by quantitative precipitation. Used as decalcifier and fixative in microscopy.

Trichloroacetic acid has been used:

• for hydrolysis of DNA isolated from the cell[5]
• for precipitation of glutathione isolated from seminal plasma[6]
• as a fixative in microscopy[7]

Safety Information

• Pictograms: GHS05,GHS09
• Signal Word: Danger
• Hazard Statements: H314,H410
• Precautionary Statements: P260 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Corr. 1A
• Storage Class Code: 8B - Non-combustible corrosive hazardous materials
• WGK: WGK 2
• Flash Point(F): >235.4 °F - closed cup
• Flash Point(C): > 113 °C - closed cup
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves