YEAST1

Yeast Transformation Kit

reagents for introducing plasmid DNA into yeast

• Synonym(s): lithium acetate yeast transformation
• UNSPSC Code: 12352200
• NACRES: NA.85

Properties

• grade: Molecular Biology
• Quality Level: 200
• usage: 1 kit sufficient for >100 standard transformations
• technique(s): transformation: suitable
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

Sigma′s Yeast Transformation Kit contains all necessary reagents and controls for efficient transformation of yeast by the lithium acetate method.

Application

Suitable for transformation of any strain of yeast. Convenient, flexible and sensitive, positive transformants can be obtained with as little as 10 ng of DNA; the optimum efficiency is in the 0.1- 3 μg range.

Features and Benefits

• Easy and ready-to-use
• Requires as little as 10 ng of plasmid DNA
• Flexibility for any strain of yeast
• Sufficient for over 100 standard transformations

Components

The Yeast Transformation Kit contains:

• Transformation Buffer; 100 ml; 100 mM lithium acetate, 10 mM Tris HCl, pH 7.6, and 1 mM EDTA
• Plate Buffer; 100 ml; 40% PEG, 100 mM lithium acetate, 10 mM Tris HCl, pH 7.5, 1 mM EDTA
• Deoxyribonucleic acid from salmon teste, 10 mg/ml; 2 x 1 ml
• Control Yeast Plasmid DNA pRS316 carrying the ura gene; 10 μg
• Yeast Synthetic Drop-out Medium Supplement Without Uracil; 1 g

Principle

Transformation with a plasmid complementing the mutated gene enables the transformant to grow on medium lacking the required component. Yeast cells are made competent for transformation by incubation in a buffered lithium acetate solution. Transformation is then carried out by incubating the cells together with transforming DNA and carrier DNA in a solution containing polyethylene glycol (PEG).

Kit Components Also Available Separately

• D9156 Deoxyribonucleic acid, single stranded from salmon testes, For hybridization 2 x 1
• Y1501 Yeast Synthetic Drop-out Medium Supplements, without uracil 1 g

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable