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G005905

HPLC Analysis of Alkaloids from Herbal Supplements on Ascentis® Express F5, HILIC Mode

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: Ascentis® Express F5, 3 cm x 3.0 mm I.D., 2.7 μm particles (53574-U)
mobile phase: 2 mM ammonium acetate in 10:90 water:acetonitrile, pH to 6.0 with acetic acid
flow rate: 0.5 mL/min
pressure: 1100 psi (76 bar)
column temp.: 35 °C
detector: UV, 275 nm
injection: 2 μL
sample: methanolic extract of Yohimbe Herbal Supplement

suitability

application for HPLC

application(s)

vitamins, nutraceuticals, and natural products

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description

    Yohimbine hydrochloride, ≥98% (HPLC), powder

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