E6412

Cellobiohydrolase I from Hypocrea jecorina

100 U/mg, recombinant, expressed in corn

• Synonym(s): Cel7A, Cellobiosidase, Cellulase
• EC Number: 3.2.1.91 (BRENDA, IUBMB)
• EC Number: 253-465-9
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• recombinant: expressed in corn
• Quality Level: 200
• form: liquid
• specific activity: 100 U/mg
• greener alternative product characteristics: Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• greener alternative category: Enabling,
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

Cellubiohydrolase I is an enzyme present in many fungi, but particularly wood rot fungi. It is a monomer of 53 kDa with a catalytic domain and a cellulose binding domain. The reaction adds water to the glucose bonds in cellulose (non-reducing ends of the chain), yielding cellobiose.

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Application

Cellobiohydrolase I can be used in combination with endocellulases and b-glucosidase to produce glucose from cellulose.

Biochem/physiol Actions

Cellobiohydrolase (CBH) is a cellulase which degrades cellulose by hydrolysing the 1,4-β-D-glycosidic bonds. CBH is an exocellulase which cleaves two to four units from the ends of cellulose. CBH I cleaves progressively from the reducing end. CBH I is commonly used in detergents for cleaning textiles. Its ezymatic activity ranges from 37° C to 50° C, with its optimal temperature being approximately 45° C. The optimum pH for the enzyme is 5-6.

Unit Definition

Unit Definition: A unit will turn over 1 nmole of methyl-umbelliferyl beta-D cellobioside per min at pH 5 at 50° C.

Physical form

Provided as an ammonium sulfate precipitate with the source as recombinant maize.

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P342 + P311
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable