SRE0095

Fast β-Glucuronidase, Recombinant

from limpets (Patella Vulgata), aqueous solution

• Synonym(s): β-Glucuronidase from limpets (Patella vulgata), β-D-Glucuronide glucuronosohydrolase
• CAS Number: 9001-45-0
• EC Number: 3.2.1.31 (BRENDA, IUBMB)
• MDL number: MFCD00130629
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• recombinant: expressed in proprietary host
• Quality Level: 200
• form: aqueous solution
• concentration: 300000-400000 units/mL
• shipped in: ambient
• storage temp.: 2-8°C

Description

Application

New Technical Article Comparing Performance of Different Enzymes
Learn more about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine

More on the suitability of this product for hydrolysis of different drug-glucuronide substrates is available at this page.

The enzyme from patella vulgata is reported to be more effective in hydrolyzing opioid-glucuronides than the Helix pomatia, bovine liver and E. coli enzymes

Features and Benefits

Formulated with a higher enzyme concentration for applications requiring ultra-fast hydrolysis rates. Recombinantly expressed and purified to eliminate MAM esterase activity (6-Monoacetylmorphine→morphine). Representative analysis at high enzyme titer (50 un/μL β-glucuronidase) was less than 1% conversion of 6-MAM to morphine after 4 hours at 60 °C.

Unit Definition

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 3.8 (30 min assay).

Physical form

Supplied as a solution in 100mM potassium acetate pH 5.2. It is free of detergents, carbohydrates or any other components that may interfere with sample preparation and analysis.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable