B6185

Bionic^™ Buffer, 10X Concentrate

for Northern and Southern blotting, solution

• Synonym(s): Sodium Borate Electrophoresis Buffer
• UNSPSC Code: 41105319
• NACRES: NA.25

Properties

• grade: Molecular Biology
• Quality Level: 200
• description: Mode of use Voltage of 150 V is recommended for small electrophoresis chambers while a mid to large apparatus may be run at 200-250 V. User should test their chamber to determine optimum voltage.
• form: solution
• concentration: 10 ×
• impurities: DNase, RNase and nickase, none detected
• pH: 8.0-8.5 (20-25 °C)
• storage temp.: room temp

Description

General description

Bionic Buffer is a unique alternative to traditional TBE (TRIS borate-EDTA) and TAE (TRIS acetate-EDTA) electrophoresis buffers. Bionic Buffer allows for higher voltage runs, extremely rapid running times, and high-resolution band separation.

Application

Suitable for:

• Use as running buffer for nucleic acid electrophoresis, which may be part of Northern and Southern blot hybridization procedures
• Use with TBA and TAE gels
• Preparation of agarose gels

Features and Benefits

• Faster run times
• Sharper bands
• Easy to dilute and use
• Flexible use with a variety of gel types

Packaging

The 4 L and 10 L sizes are supplied in dispensers with a spigot.

Caution

Test electrophoresis chambers to determine highest voltage. Voltages beyond the recommended range for your apparatus may result in blown fuses or damage to your apparatus.

Legal Information

Bionic is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• Pictograms: GHS08,GHS07
• Signal Word: Danger
• Hazard Statements: H319,H360FD
• Precautionary Statements: P202 - P264 - P280 - P305 + P351 + P338 - P308 + P313 - P337 + P313
• Hazard Classifications: Eye Irrit. 2 - Repr. 1B
• Storage Class Code: 6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves