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F9786

Sigma-Aldrich

Fibroblast Growth Factor-Basic Heparin Stabilized human

recombinant, expressed in E. coli, aqueous solution, suitable for cell culture

Synonym(s):

rhbFGF

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About This Item

CAS Number:
106096-93-9
MDL number:
MFCD00081382
UNSPSC Code:
12352202
NACRES:
NA.77
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Product Name

Fibroblast Growth Factor-Basic Heparin Stabilized human, FGF-Basic, recombinant, expressed in E. coli, sterile-filtered, aqueous solution, suitable for cell culture

biological source

human

Quality Level

300

recombinant

expressed in E. coli

sterility

sterile-filtered

form

aqueous solution

potency

0.03-3.0 ng/mL ED50/EC50

quality

endotoxin tested

packaging

pkg of 1 and 5 mL

technique(s)

cell culture | mammalian: suitable

Related Categories

General description

Fibroblast growth factor 2 (FGF2), a canonical member, belongs to the fibroblast growth factor family.[1]

Application

Fibroblast Growth Factor-Basic Heparin Stabilized human has been used to culture Brown Norway rat yolk sac epithelial cells transformed with mouse sarcoma virus (BN/MSV).[2] It has also been used in the preparation of serum-free mouse tubal epithelium growth medium (M-TE-GM) to culture primary tubal epithelium (TE) cells.[3]

Biochem/physiol Actions

Fibroblast growth factor 2 (FGF2) participates in tissue development and repair.[1] It is also involved in embryonic development, angiogenesis, bone regeneration, stem cell self-renewal, and wound healing. The basic fibroblast growth factor (bFGF) plays a key role in the progression and maintenance of the nervous system.[4] FGF2 binds to the FGF receptor (FGFR) and activates the extracellular signal-regulated kinase (ERK) pathway, which controls cellular behavior, using heparan sulfates (HS)/heparin as a cofactor.[1] bFGF stimulates cell proliferation, differentiation, and migration in a variety of cell types.[4] In cultured astrocytes from rat brain, recombinant human basic fibroblast growth factor (rhbFGF) may induce nerve growth factor (NGF) secretion.[5] It is the heparin stabilized form of a natural mitogenic growth factor used in cell culture applications to study cell signaling and to promote fibroblast and endothelial cell growth and survival.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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    Certificates of Analysis (COA)

    Lot/Batch Number
    SLBZ3354
    SLBX8558
    SLBW1411
    SLBT0889
    SLBQ4559V

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    Michele Galluccio et al.
    Molecular biology reports, 47(9), 7283-7289 (2020-08-11)
    It is well established that Escherichia coli represents a powerful tool for the over-expression of human proteins for structure/function studies. In many cases, such as for membrane transporters, the bacterial toxicity or the aggregation of the target protein hamper the
    Lucie Peterková et al.
    Tissue & cell, 58, 121-129 (2019-05-28)
    Surface modification is an important step in making a synthetic polymer cytocompatible. We have previously reported improved cytocompatibility of immortalized human keratinocytes (HaCaT) with the otherwise bioinert fluorinated ethylene propylene (FEP) upon treatment with argon plasma discharge. In this article

    Protocols

    Human Dermal Fibroblasts (HDF) Culture Protocol

    Storage - Preparation for Culturing - Culturing HDF - Subculturing HDF

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