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G6048

Sigma-Aldrich

Galactokinase human

recombinant, expressed in E. coli

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200 μG
RM 2,190.00

RM 2,190.00

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200 μG
RM 2,190.00

About This Item

EC Number:
2.7.1.6 (BRENDA, IUBMB)
UNSPSC Code:
12352204
NACRES:
NA.54

RM 2,190.00

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recombinant

expressed in E. coli

Quality Level

200

form

solution

specific activity

≥1400 unit/μg protein

mol wt

42 kDa

shipped in

dry ice

storage temp.

−70°C

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Galactokinase human recombinant, expressed in E. coli

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Galactokinase human

Hck human recombinant, expressed in E. coli BL21, vial of ≥200 units

H1038

Hck human

Cyclin-Dependent Kinase 5 human recombinant, expressed in E. coli BL21, 50-100 units/μg protein

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Cyclin-Dependent Kinase 5 human

Acetyl-CoA carboxylase 2 human recombinant, expressed in Sf9 cells

A6861

Acetyl-CoA carboxylase 2 human

specific activity

≥1400 unit/μg protein

specific activity

1-2 unit/μg protein

specific activity

50-100 units/μg protein

specific activity

≥25 units/μg protein

form

solution

form

solution

form

solution

form

solution

recombinant

expressed in E. coli

recombinant

expressed in E. coli BL21

recombinant

expressed in E. coli BL21

recombinant

expressed in Sf9 cells

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

mol wt

42 kDa

mol wt

-

mol wt

-

mol wt

-

Biochem/physiol Actions

Galactokinase catalyzes the phosphorylation of αD-galactose to produce galactose-1-phosphate as part of the Leloir pathway[1].

Physical properties

N-terminal GST-tagged 42 kDa full length protein

Unit Definition

One unit will convert 1.0 picomole of galactose to galactose-1-phosphate per minute at pH 7.4 at 30 °C.

Physical form

Supplied as a solution in 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, 3 mM DTT and 10-250 mM imidazole.

Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Danger

Hazard Statements

H315,H319,H360D

Hazard Classifications

Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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    Certificates of Analysis (COA)

    Lot/Batch Number
    SLCH4725
    SLBH6889V
    SLBH2942V
    SLBF2096V
    SLBD9958V

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    P A Frey
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 10(4), 461-470 (1996-03-01)
    The biological interconversion of galactose and glucose takes place only by way of the Leloir pathway and requires the three enzymes galactokinase, galactose-1-P uridylyltransferase, and UDP-galactose 4-epimerase. The only biological importance of these enzymes appears to be to provide for
    Chieh Hsu et al.
    Nature communications, 3, 682-682 (2012-02-23)
    During evolution, genetic networks are rewired through strengthening or weakening their interactions to develop new regulatory schemes. In the galactose network, the GAL1/GAL3 paralogues and the GAL2 gene enhance their own expression mediated by the Gal4p transcriptional activator. The wiring
    Sanjay K Upadhyay et al.
    Journal of computer-aided molecular design, 26(7), 847-864 (2012-05-29)
    The Gal4p mediated transcriptional activation of GAL genes requires the interaction between Gal3p bound with ATP and galactose and Gal80p. Though numerous studies suggest that galactose and ATP activate Gal3p/Gal1p interaction with Gal80p, neither the mechanism of activation nor the
    Kajal Biswas et al.
    Methods in molecular biology (Clifton, N.J.), 852, 121-131 (2012-02-14)
    Recombineering is a recombination-based highly efficient method of genetic engineering. It can be used to manipulate the bacterial chromosomal DNA as well as any episomal DNA. Recombineering can be used to insert selectable or nonselectable DNA fragments and subclone DNA
    Ling Lin et al.
    Proceedings of the National Academy of Sciences of the United States of America, 109(6), 1997-2002 (2012-02-07)
    Promoter-specific transcriptional activators (activators) stimulate transcription through direct interactions with one or more components of the transcription machinery, termed the "target." The identification of direct in vivo targets of activators has been a major challenge. Previous studies have provided evidence
    View All Related Papers

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