biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
MEM-E/08, monoclonal
form
buffered aqueous solution
species reactivity
human
concentration
1 mg/mL
technique(s)
flow cytometry: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... HLA-E(3133)
General description
The antibody MEM-E/08 recognized native surface-expressed HLA-E, but not denaturated heavy chain of HLA-E. HLA-E belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on many types of the human cells. The published results revealed that antibody is remarkably specific for HLA-E, only with weak cross-reactivity with following classical MHC Class I molecules (MHC Class Ia): HLA-A24, -B7, -B27, -B51, -B54, -C7.
Immunogen
Bacterially expressed recombinant HLA-E refolded with beta2-microglobulin and peptide
Application
The reagent is designed for Flow Cytometry analysis. Suggested working dilution for Flow Cytometry is 4 μg/mL of sample. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.
Features and Benefits
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Physical form
Solution in phosphate buffered saline, pH 7.4, with 15 mM sodium azide.
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Storage Class Code
10 - Combustible liquids
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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