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G005735

HPLC Analysis of Water Soluble Vitamins on Ascentis® RP-Amide

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: Ascentis RP-Amide, 15 cm x 4.6 mm I.D., 5 μm particles (565324-U)
mobile phase: 25 mM potassium phosphate monobasic, pH 3.5
flow rate: 1.0 mL/min
column temp.: 30 °C
detector: UV, 230 nm
injection: 10 μL

suitability

application for HPLC

application(s)

vitamins, nutraceuticals, and natural products

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Nicotinic acid
    L-Ascorbic acid
    Pyridoxine

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