Skip to Content
Merck
  • Multipotent adult hippocampal progenitor cells maintained as neurospheres favor differentiation toward glial lineages.

Multipotent adult hippocampal progenitor cells maintained as neurospheres favor differentiation toward glial lineages.

Biotechnology journal (2014-05-23)
Jisun Oh, Gabrielle J Daniels, Lawrence S Chiou, Eun-Ah Ye, Yong-Seob Jeong, Donald S Sakaguchi
ABSTRACT

Adult hippocampal progenitor cells (AHPCs) are generally maintained as a dispersed monolayer population of multipotent neural progenitors. To better understand cell-cell interactions among neural progenitors and their influences on cellular characteristics, we generated free-floating cellular aggregates, or neurospheres, from the adherent monolayer population of AHPCs. Results from in vitro analyses demonstrated that both populations of AHPCs were highly proliferative under maintenance conditions, but AHPCs formed in neurospheres favored differentiation along a glial lineage and displayed greater migrational activity than the traditionally cultured AHPCs. To study the plasticity of AHPCs from both populations in vivo, we transplanted green fluorescent protein (GFP)-expressing AHPCs via intraocular injection into the developing rat eyes. Both AHPC populations were capable of surviving and integrating into developing host central nervous system, but considerably more GFP-positive cells were observed in the retinas transplanted with neurosphere AHPCs, compared to adherent AHPCs. These results suggest that the culture configuration during maintenance for neural progenitor cells (NPCs) influences cell fate and motility in vitro as well as in vivo. Our findings have implication for understanding different cellular characteristics of NPCs according to distinct intercellular architectures and for developing cell-based therapeutic strategies using lineage-committed NPCs.

MATERIALS
Product Number
Brand
Product Description

SAFC
Sodium chloride solution, 5 M
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Sodium chloride solution, 0.9% in water, BioXtra, suitable for cell culture
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Sodium chloride, DNase, RNase, and protease, none detected, ≥99% (titration)
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Sodium chloride, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Sodium chloride, BioPerformance Certified, ≥99% (titration), suitable for insect cell culture, suitable for plant cell culture
SKU
Pack Size
Availability
Price
Quantity
Supelco
Sodium chloride, Pharmaceutical Secondary Standard; Certified Reference Material
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Sodium chloride, BioXtra, ≥99.5% (AT)
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Sodium chloride, tested according to Ph. Eur.
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Sodium chloride, BioUltra, ≥99.5% (AT)
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Sodium chloride solution, 5 M in H2O, BioReagent, suitable for cell culture
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Sodium chloride, 99.999% trace metals basis
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Sodium chloride solution, BioUltra, ~5 M in H2O
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Sodium chloride solution, 5 M
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Sodium chloride-35Cl, 99 atom % 35Cl
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Sodium chloride solution, 0.85%
SKU
Pack Size
Availability
Price
Quantity
Supelco
Sodium chloride, reference material for titrimetry, certified by BAM, >99.5%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Sodium chloride, meets analytical specification of Ph. Eur., BP, USP, 99.0-100.5%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Sodium chloride, tablet
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Sodium chloride, AnhydroBeads, −10 mesh, 99.999% trace metals basis
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Sodium chloride, random crystals, optical grade, 99.9% trace metals basis
SKU
Pack Size
Availability
Price
Quantity
Supelco
L-Glutamine, Pharmaceutical Secondary Standard; Certified Reference Material
SKU
Pack Size
Availability
Price
Quantity
Supelco
L-Glutamine, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
L-Glutamine, BioUltra, ≥99.5% (NT)
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
L-Glutamine, ReagentPlus®, ≥99% (HPLC)
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
L-Glutamine, meets USP testing specifications, suitable for cell culture, 99.0-101.0%, from non-animal source
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
L-Glutamine, γ-irradiated, BioXtra, suitable for cell culture
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
DAPI, for nucleic acid staining
SKU
Pack Size
Availability
Price
Quantity
SAFC
L-Glutamine
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
L-Glutamine
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
FGF-2 human, recombinant, expressed in insect cells, ≥85% (SDS-PAGE)
SKU
Pack Size
Availability
Price
Quantity