Characterization of ceramic hydroxyapatite surface by inverse liquid chromatography in aquatic systems.

Characterization of ceramic hydroxyapatite surface by inverse liquid chromatography in aquatic systems.

Talanta (2015-11-26)

Karol Kadlec, Katarzyna Adamska, Adam Voelkel

ABSTRACT

The novel approach for hydroxyapatite (HA) surface characterization was proposed. The main aim of this investigation was to estimate surface properties of HA as a biomaterial in real system i.e. in simulated body fluid (SBF). One of the method, which might be used to reflect the influence of liquid environment on sorption properties of material being surrounded by this liquid, is called inverse liquid chromatography (ILC). The lowercase letters of LFER equation (e, s, a, b, v) served for this characterization. The sorption abilities of examined material were also estimated for two different aqueous mobile phases: deionized water and water solution of 0.1M Na2HPO4. It enabled to observe the change in physiochemical properties of surface, considered in Abraham model, dependence on ions concentration in the mobile phase. Moreover pH of every aquatic solution, normally about 7, was adjusted to 5.5 and 9 to observe the influence of hydrogen and hydroxyl ions concentration on HA sorption properties.

MATERIALS

Product Number

Brand

Product Description

59222C

SAFC

Sodium chloride solution, 5 M

279544

Sigma-Aldrich

1-Propanol, anhydrous, 99.7%

W200603

Sigma-Aldrich

Acetic acid, natural, ≥99.5%, FG

W200611

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109819

Sigma-Aldrich

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451940

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240958

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Propylamine, ≥99%

606766

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Acetic acid-¹²C₂, 99.9 atom % ¹²C

574732

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Acetonitrile solution, contains 0.1 % (v/v) trifluoroacetic acid, suitable for HPLC

574724

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Acetonitrile solution, contains 0.05 % (v/v) trifluoroacetic acid

576956

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Acetonitrile solution, contains 0.1 % (v/v) formic acid, suitable for HPLC

S6297

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Sodium bicarbonate, BioXtra, 99.5-100.5%

C5267

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Calcium phosphate tribasic, 34.0-40.0% Ca basis

450006

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693863

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13464

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60349

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93362

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33209

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Acetic acid, glacial, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.8%

45726

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Acetic acid, BioUltra, ≥99.5% (GC)

900506

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Sodium bicarbonate, anhydrous, free-flowing, Redi-Dri^™, ReagentPlus^®, ≥99.5%

13433

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Sodium bicarbonate, puriss., meets analytical specification of Ph. Eur., BP, USP, FCC, E500, 99.0-100.5%, powder

451029

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574791

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04238

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793590

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746436

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449989

Sigma-Aldrich

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