Clusterin is also known as apolipoprotein J, complement lysis inhibitor, gp80, glycoprotein III, SGP-2, SP 40-40, TRPM2 and T64. It is a ubiquitous multifunctional protein of 80kD comprising of two disulfide-linked subunits, alpha and beta, and capable of interacting with a broad range of molecules. It is implicated in numerous biological processes including sperm maturation, lipid transport, regulation of the complement cascade, membrane recycling, cell death, immune regulation, cell adhesion and morphological transformation. In pathological conditions, it is an amyloid associated protein co-localising with fibrillar deposits in systemic and localised amyloid disorders. In Alzheimer ’s disease, clusterin is present in amyloid plaques and cerebrovascular deposits. In breast cancers, clusterin expression was found to correlate with tumour size. Clusterin is also implicated with endometrial cancers, myocardial infarction, renal diseases and atherosclerosis.

This antibody recognizes human clusterin α chain, Mr 40 kDa, and precursor protein.

Native clusterin purified from human serum. Clone 41D.

Anti-Clusterin α chain (human) Antibody, clone 41D is an antibody against Clusterin α chain (human) for use in IC, IH, IH(P) & WB.

Immunohistochemistry:
Previous lots of this antibody at 5 µg/mL detected clusterin in paraffin embedded human brain sections.

Research Category
Apoptosis & Cancer

Neuroscience

Research Sub Category
Apoptosis - Additional

Neurodegenerative Diseases

Routinely evaluated on RIPA lysates from HeLa cells or MCF-7 cell lysates.

Western Blot Analysis:
0.5-2 µg/mL of a previous lot detected human clusterin α chain in RIPA lysates from HeLa cells; previous lots detected the protein in human serum and MCF-7 cell lysates

40 kDa

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, with 0.05% sodium azide. Frozen at -20°C.

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Control
HeLa cell lysate.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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