06-747
Anti-Neomycin Phosphotransferase II Antibody
Upstate®, from rabbit
동의어(들):
Anti-Neomycin II, Neomycin II Antibody, Neomycin Phosphotransferase Ab
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About This Item
UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41
제품 06-747은(는)귀하의 국가에서 현재 판매되지 않습니다. 고객지원팀으로 연락바랍니다.
추천 제품
생물학적 소스
rabbit
Quality Level
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
polyclonal
종 반응성
human, bacteria
종 반응성(상동성에 의해 예측)
mouse, rat
제조업체/상표
Upstate®
기술
western blot: suitable
동형
IgG
1 of 4
이 품목 | MABE343-AF647 | MABE343 | MABE341 |
|---|---|---|---|
| species reactivity human, bacteria | species reactivity human | species reactivity human | species reactivity human |
| clone polyclonal | clone 12D10, monoclonal | clone 12D10, monoclonal | clone 17H1, monoclonal |
| biological source rabbit | biological source mouse | biological source mouse | biological source rat |
| antibody form purified immunoglobulin | antibody form purified antibody | antibody form purified immunoglobulin | antibody form purified antibody |
| UniProt accession no. | UniProt accession no. - | UniProt accession no. - | UniProt accession no. - |
| technique(s) western blot: suitable | technique(s) immunocytochemistry: suitable | technique(s) flow cytometry: suitable, immunocytochemistry: suitable, immunofluorescence: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable | technique(s) western blot: suitable |
일반 설명
Neomycin phosphotransferase II confers resistance to kanamycin and neomycin in bacteria and G418 (Geneticin®, G418 Sulfate) in mammalian cells. These antibiotics bind to ribosomal components and inhibit protein synthesis. NPT II inhibits these antibiotics through phosphorylation, which is thought to interfere with their active transport into the cell. Immunoblot analysis of transfected cell lysates with anti NPT II can be used to gauge relative transfection efficiencies in experiments where multiple transfections are performed in parallel with different plasmids.
특이성
Recognizes NPT II, Mr ~30 kDa
면역원
Full length neomycin phosphotransferase (NPT) II
애플리케이션
Anti-Neomycin Phosphotransferase II Antibody is an antibody against Neomycin Phosphotransferase II for use in WB.
Research Category
Epitope Tags & General Use
Epitope Tags & General Use
Research Sub Category
Epitope Tags
Epitope Tags
Western Blot Analysis:
1-2 μg/mL dilutions of a previous lot detected NPT II in RIPA lysates from Cos-1 cells transfected with pUSE, harboring the neomycin resistance gene.
1-2 μg/mL dilutions of a previous lot detected NPT II in RIPA lysates from Cos-1 cells transfected with pUSE, harboring the neomycin resistance gene.
품질
Routinely evaluated by Western Blot on transiently transfected Cos-1 cells.
Western Blot Analysis:
1-2 μg/mL of a previous lot detected NPT II in RIPA lysate from Cos-1 cells transfected with pUSE, harboring the neomycin resistance gene.
Western Blot Analysis:
1-2 μg/mL of a previous lot detected NPT II in RIPA lysate from Cos-1 cells transfected with pUSE, harboring the neomycin resistance gene.
표적 설명
~30 kDa
물리적 형태
Format: Purified
Protein A purified
Purified rabbit polyclonal IgG in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide before the addition of glycerol to 30%. Liquid at -20°C.
저장 및 안정성
Stable for 1 year at 2 - 8°C from date of receipt.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at 2 - 8°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below 2 - 8°C may cause glycerol containing solutions to become frozen during storage.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at 2 - 8°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below 2 - 8°C may cause glycerol containing solutions to become frozen during storage.
분석 메모
Control
RIPA lysates from Cos-1 cells transfected with pUSE, harboring the neomycin resistance gene
RIPA lysates from Cos-1 cells transfected with pUSE, harboring the neomycin resistance gene
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
Geneticin is a registered trademark of Gibco BRL Life Technologies, Inc.
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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