Name: Tris Base, Molecular Biology Grade
Brand: MM

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About This Item

Linear Formula:

NH₂C(CH₂OH)₃

CAS Number:

77-86-1

Molecular Weight:

121.14

Beilstein:

741883

MDL number:

MFCD00004679

UNSPSC 코드:

12161705

NACRES:

NA.25

기술 서비스

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도움 문의

Product Name

Tris Base, Molecular Biology Grade, TRIS base is useful in the pH range of 7.0-9.0. Has a pKa of 8.1 at 25°C.

Grade

Molecular Biology

Quality Level

100

설명

Merck USA index - 14, 9772

분석

≥99% (perchloric acid titration)

양식

solid

제조업체/상표

Calbiochem^®

저장 조건

OK to freeze

색상

white

유용한 pH 범위

7-9

pKa(25 °C)

8.1

bp

219-220 °C/10 mmHg (lit.)

mp

167-172 °C (lit.)

solubility

water: 500 mg/mL

양이온 미량물

heavy metals: ≤0.0005%

흡수

≤0.02 at 280 nm
≤0.03 at 260 nm

외래 활성

RNases, DNases, proteases, none detected

저장 온도

10-30°C

SMILES string

NC(CO)(CO)CO

InChI

1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2

InChI key

LENZDBCJOHFCAS-UHFFFAOYSA-N

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일반 설명

Useful in the pH range of 7.0-9.0. Has a pK_a of 8.1 at 25°C.

Widely used as a biological buffer (absorbance: ≤0.03 at 260 nm; ≤0.02 at 280 nm). Useful in the pH range of 7.0-9.0. Has a pK_a of 8.1 at 25°C.

애플리케이션

A Fast and Automated Melanin-bleaching Method for Histopathologic Evaluation of Pigmented Melanoma Tissues.: This study presents an automated melanin-bleaching technique using a Tris-based buffer to enhance histopathologic evaluation of pigmented melanoma tissues, aiding in more accurate diagnoses (Ugolini et al., 2022).

Improvement of plant protein solubilization and 2-DE gel resolution through optimization of the concentration of Tris in the solubilization buffer.: Highlights the optimization of Tris concentration in solubilization buffers to enhance protein solubilization and gel electrophoresis resolution, crucial for biochemical studies (Cho et al., 2010).

경고

Toxicity: Irritant (B)

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)