Interleukin-1 beta (UniProt; also known as IL-1 beta, IL-1b, IL-1beta) is encoded by the Il1b gene (Gene ID 16176) in murine species. Interleukin-1 (IL-1) is a cytokine that plays a central role in the regulation of acute and chronic inflammation. Two forms of IL-1, IL-1 and IL-1 , are transcribed from two separate genes and mediate similar, but not completely overlapping, biological effects via the membrane-bound IL-1 type 1 receptor (IL-1R1). Studies utilizing Il1a- and Il1b-knockout mice showed that IL-1 , but not IL-1 , is involved in T-cell-dependent antibody production via antigen-specific T-cell activation. IL-1 binding to the extracellular domain of IL1R1 promotes the recruitment of the IL-1 receptor accessory protein and subsequent signal transduction mediated by the cytoplasmic domains of IL-1R1 and IL1-R2. The IL-1 type 2 receptor (IL1R2) is unable to initiate signaling and only acts as a decoy receptor. Soluble receptors for IL-1 (sIL-1Rs) are produced by proteolytic cleavage of membrane-bound receptors or by alternative splicing and act as natural inhibitors by competing against membrane-bound IL1Rs for IL-1 binding. Murine IL-1 beta is produced with a propeptide sequence (a.a. 1-117), which is removed posttranslationally to yield the mature (a.a. 118-269) protein.

The unconjugated antibody (Cat. No. AB1413-I) detected both the ~17 kDa mature and ~30 kDa pro-form of murine IL-1beta. The corresponding sequence in human IL-1beta differs from that of the immunogen by only one amino acid. Immunogen sequence is not found in IL-1alpha.

KLH-conjugated linear peptide corresponding to a sequence within the C-terminal half of murine and rat mature IL-1beta.

Research Category
Inflammation & Immunology

The unconjugated antibody (Cat. No. AB1413-I) and Alexa Fluor^™ 555 conjugate (Cat. No. AB1413-I-AF555) are also available for Immunocytochemistry, Immunoprecipitation, and Western Blotting applications.

This rabbit polyclonal Anti-Interleukin-1 Antibody, Alexa Fluor^™ 488 Conjugate, Cat. No. AB1413-I-AF488, is validated for use in Immunocytochemistry for the detection of Interleukin-1 .

Evaluated by Immunocytochemistry in NIH/3T3 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected IL-1beta in 4% paraformaldehyde-fixed, Triton X-100-permeabilized NIH/3T3 cells.

~17/30 kDa observed. 17.39/30.93 kDa (mouse mature/pro-form), 17.32/30.64 kDa (rat mature/pro-form) calculated. Uncharacterized bands may be observed in some lysate(s).

Affinity purified.

Purified rabbit polyclonal antibody conjugate in PBS with 1.5% BSA and 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to lot specific datasheet.

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