Lymphocyte cytosolic protein 2 (UniProt Q13094; also known as SH2 domain-containing leukocyte protein of 76 kDa, SLP-76 tyrosine phosphoprotein, SLP76) is encoded by the LCP2 gene (Gene ID 3937) in human. SLP76 is a signaling adaptor molecule expressed in a variety of hematopoietic cells, including lymphocytes, myeloid cells, and platelets. SLP-76 contains three pYESP motifs (Y113 and Y128) that are phosphorylated by ZAP-70 to produce docking sites for SH2-containing proteins, such as exchange factor Vav1, adaptor Nck, resting lymphocyte kinase Rlk, and inducible tyrosine kinase Itk. In addition, SLP-76 binds to the SH3 domain of phospholipase Cγ1 (PLCγ1) and SLP76-deficient T cells show an impaired PLCγ1 activation and calcium mobilization. SLP-76 is essential for mediating TCR signaling, Lcp2-/- mice exhibit impaired thymic development and their T cells fail to develop beyond stage III at the double-negative stage. SLP76 is also a critical determinant of NK-cell development and the the underlying “missing-self” recognition mechanism that prevents NK cells from destroying cells that express self major histocompatibility complex (MHC) class I molecules. SLP76 contains an N-terminal sterile-α motif or SAM (human a.a. 15-81 and moust a.a. 12-78) and a C-terminal SH2 domain (human/mouse a.a. 422-530).

This rabbit polyclonal antibody targets a central region sequence within the N-terminal half of human SLP76.

KLH-conjugated linear peptide corresponding to a central region sequence within the N-terminal half of human SLP76.

Immunoprecipitation Analysis: 10 µL from a representative lot immunoprecipitated SLP76 from 500 µg of Jurkat cell lysate.

Research Category
Inflammation & Immunology

This rabbit polyclonal Anti-SLP76, Cat. No. ABF1215 is validated for use in Immunoprecipitation and Western Blotting for the detection of SLP76.

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected SLP76 in 10 µg of Jurkat cell lysate.

~76 kDa observed. 60.19 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Replaces: 06-548

Affinity purified.

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to lot specific datasheet.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.