생물학적 소스
rabbit
항체 형태
affinity isolated antibody
항체 생산 유형
primary antibodies
클론
polyclonal
정제법
affinity chromatography
종 반응성
human, mouse
포장
antibody small pack of 25 μg
기술
immunocytochemistry: suitable
western blot: suitable
NCBI 수납 번호
UniProt 수납 번호
타겟 번역 후 변형
unmodified
유전자 정보
human ... SCYL1(57410)
일반 설명
N-terminal kinase-like protein (UniProt: Q96KG9; also known as Coated vesicle-associated kinase of 90 kDa, SCY1-like protein 1, Telomerase regulation-associated protein, Telomerase transcriptional element-interacting factor, Teratoma-associated tyrosine kinase) is encoded by the SCYL1 (also known as CVAK90, GKLP, NTKL, TAPK, TEIF, TRAP, HT019) gene (Gene ID: 57410) in human. SCY1-like protein 1 is ubiquitously expressed and it regulates COPI-mediated retrograde protein traffic at the interface between the Golgi apparatus and the endoplasmic reticulum. It is involved in the maintenance of the Golgi apparatus morphology. It has a divergent N-terminal kinase domain (aa 14-314), but it is catalytically inactive. It also contain three HEAT repeats (aa 350-388; 389-427; and 507-545). Six isoforms of SCY1-like protein 1 have been reported that are produced by alternative splicing. Its intracellular distribution is isoform specific. Isoforms 1 and 2 are shown to be cytoplasmic throughout the cell cycle. Isoform 3 is cytoplasmic during interphase and centrosomal during mitosis. Isoform six is generally localized to nucleus and it acts as a transcriptional activator and binds to three different types of GC-rich DNA binding sites (box-A, -B and -C) in the beta-polymerase promoter region. Mutations in SCYL1 gene are reported to cause Spinocerebellar ataxia, a clinically and genetically heterogeneous group of cerebellar disorders due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord.
특이성
This rabbit polyclonal antibody detects SCY1-Like Protein 1 (SCYL1) in human cells. It targets an epitope within 16 amino acids from the C-terminal region. The immunogen is conserved between isoforms 1, 2, and 3.
면역원
Epitope: C-terminus
KLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal region of human SCY1-Like Protein 1.
애플리케이션
Anti-SCYL1, Cat. No. ABS1494, is a rabbit polyclonal antibody that detects SCY1-Like Protein 1 and has been tested for use in Immunocytochemistry and Western Blotting.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected SCYL1 in HeLa, A431, HUVEC, and NIH/3T3 cells.
Research Category
Signaling
Signaling
품질
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 0.2 µg/mL of this antibody detected SCY1-Like Protein 1 (SCYL1) in HeLa cell lysate.
Western Blotting Analysis: 0.2 µg/mL of this antibody detected SCY1-Like Protein 1 (SCYL1) in HeLa cell lysate.
표적 설명
~100 kDa observed; 89.63 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
물리적 형태
Affinity Purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
저장 및 안정성
Stable for 1 year at 2-8°C from date of receipt.
기타 정보
Concentration: Please refer to lot specific datasheet.
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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