LP02

Anti-LDL Receptor Mouse mAb (15C8)

liquid, clone 15C8, Calbiochem^®

• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: 15C8, monoclonal
• 양식: liquid
• 포함: ≤0.1% sodium azide as preservative
• 종 반응성: human, bovine
• 반응하면 안 됨: rabbit
• 제조업체/상표: Calbiochem^®
• 저장 조건: do not freeze
• 동형: IgG2a
• 배송 상태: wet ice
• 저장 온도: 2-8°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... LDLR(3949)

설명

일반 설명

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with Sp2/0-Ag14 myeloma cells (see application references). Recognizes the ~160 kDa LDL Receptor protein.

Recognizes the ~160 kDa LDL receptor protein in HT1080 and HepG2 cells.

This Anti-LDL Receptor Mouse mAb (15C8) is validated for use in ELISA, Flow Cytometry, Frozen Sections, Immunoblotting, Immunocytochemistry, Immunoprecipitation for the detection of LDL Receptor.

면역원

Bovine

Epitope: first cysteine-rich ligand binding domain

a trypic digest of bovine low density lipoprotein receptor

애플리케이션

ELISA (0.5-1 g/ml)
Flow Cytometry (see application references)
Frozen Sections (1-5 g/ml)
Immunoblotting (1-5 g/ml, see application references)
Immunocytochemistry (1-5 g/ml, IF)
Immunoprecipitation (1-2 g/sample)

포장

Please refer to vial label for lot-specific concentration.

경고

Toxicity: Standard Handling (A)

물리적 형태

In 0.05 M sodium phosphate buffer, 0.2% gelatin.

분석 메모

Positive Control
Liver sections, HT1080 cells, HepG2 cells

기타 정보

Will displace 50-70% of LDL binding to the receptor. For immunoblotting, include 2 mM Ca⁺² with the antibody and avoid EDTA. This antibody has been reported to bind best to the unreduced LDL-R (see specificity application reference). Antibody should be titrated for optimal results in individual systems.

van Driel, I.R., et al. 1987. J. Biol. Chem.262, 16127.
van Driel, I.R., et al. 1987. J. Biol. Chem.262, 17443.
Russell, D.W., et al. 1984. Cell37, 577.
Achneider, W.J., et al. 1983. J. Cell Biol.97, 1635.
Cummings, R.D., et al. 1983. J. Biol. Chem.258, 15261.
Schneider, W.J., et al. 1982 J. Biol. Chem.257, 2664.
Goldstein, J.L., et al. 1979. Nature279, 679.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: nwg