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LP02

Sigma-Aldrich

Anti-LDL Receptor Mouse mAb (15C8)

liquid, clone 15C8, Calbiochem®

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About This Item

UNSPSC 코드:
12352203
NACRES:
NA.41
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기술 서비스
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도움 문의

생물학적 소스

mouse

Quality Level

항체 형태

purified antibody

항체 생산 유형

primary antibodies

클론

15C8, monoclonal

양식

liquid

포함

≤0.1% sodium azide as preservative

종 반응성

human, bovine

반응하면 안 됨

rabbit

제조업체/상표

Calbiochem®

유사한 품목 비교

전체 비교 보기

차이점 표시

1 of 4

이 품목
WHA10370111WHA10370105WHA10370172
manufacturer/tradename

Whatman 10370119, Whatman Article No. 28414768 (US reference)

manufacturer/tradename

Whatman 10370111, Whatman Article No. 28414767 (US reference)

manufacturer/tradename

Whatman 10370105, Whatman Article No. 28414766 (US reference)

manufacturer/tradename

Whatman 10370172, Whatman Article No. 28414772 (US reference)

pore size

~3.0 μm (Grade GF 8)

pore size

~3.0 μm (Grade GF 8)

pore size

~3.0 μm (Grade GF 8)

pore size

~3.0 μm (Grade GF 8)

diam.

47 mm

diam.

200 mm

diam.

90 mm

diam.

-

material

circles white glass membrane (with inorganic binder)

material

circles white glass membrane (with inorganic binder)

material

circles white glass membrane (with inorganic binder)

material

sheets white glass microfiber filter (with inorganic binder)

parameter

Fast flow rate

parameter

Fast flow rate

parameter

Fast flow rate

parameter

Fast flow rate

sterility

non-sterile

sterility

-

sterility

-

sterility

non-sterile

일반 설명

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with Sp2/0-Ag14 myeloma cells (see application references). Recognizes the ~160 kDa LDL Receptor protein.
Recognizes the ~160 kDa LDL receptor protein in HT1080 and HepG2 cells.
This Anti-LDL Receptor Mouse mAb (15C8) is validated for use in ELISA, Flow Cytometry, Frozen Sections, Immunoblotting, Immunocytochemistry, Immunoprecipitation for the detection of LDL Receptor.

면역원

Bovine
Epitope: first cysteine-rich ligand binding domain
a trypic digest of bovine low density lipoprotein receptor

애플리케이션


ELISA (0.5-1 g/ml)
Flow Cytometry (see application references)
Frozen Sections (1-5 g/ml)
Immunoblotting (1-5 g/ml, see application references)
Immunocytochemistry (1-5 g/ml, IF)
Immunoprecipitation (1-2 g/sample)

포장

Please refer to vial label for lot-specific concentration.

경고

Toxicity: Standard Handling (A)

물리적 형태

In 0.05 M sodium phosphate buffer, 0.2% gelatin.

분석 메모

Positive Control
Liver sections, HT1080 cells, HepG2 cells

기타 정보

Will displace 50-70% of LDL binding to the receptor. For immunoblotting, include 2 mM Ca+2 with the antibody and avoid EDTA. This antibody has been reported to bind best to the unreduced LDL-R (see specificity application reference). Antibody should be titrated for optimal results in individual systems.
van Driel, I.R., et al. 1987. J. Biol. Chem.262, 16127.
van Driel, I.R., et al. 1987. J. Biol. Chem.262, 17443.
Russell, D.W., et al. 1984. Cell37, 577.
Achneider, W.J., et al. 1983. J. Cell Biol.97, 1635.
Cummings, R.D., et al. 1983. J. Biol. Chem.258, 15261.
Schneider, W.J., et al. 1982 J. Biol. Chem.257, 2664.
Goldstein, J.L., et al. 1979. Nature279, 679.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

10 - Combustible liquids

WGK

nwg


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