MAB308

Anti-Dopamine β Hydroxylase Antibody, clone 4F10.2

ascites fluid, clone 4F10.2, Chemicon^®

• 동의어(들): DBH
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: ascites fluid
• 항체 생산 유형: primary antibodies
• 클론: 4F10.2, monoclonal
• 종 반응성: rat, bovine, sheep
• 제조업체/상표: Chemicon^®
• 기술: immunofluorescence: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
• 동형: IgG1
• NCBI 수납 번호: NM_000787.3
• UniProt 수납 번호: P09172
• 배송 상태: dry ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: bovine ... Dbh(280758) ; rat ... Dbh(25699)

설명

일반 설명

Dopamine Beta Hydroxylase (DBH), the structural locus encoding DβH enzyme, is a major quantitative trait locus influencing plasma DβH activity, with one single nucleotide polymorphism (SNP) accounting for up to 50% of the variance. Mutations at DBH appear to be responsible for the rare syndrome of DβH deficiency. It functions as the only enzyme involved in the synthesis of small-molecule neurotransmitters that is membrane-bound, making norepinephrine and epinephrine the only transmitters synthesized inside vesicles. It is expressed in noradrenergic nerve terminals of the central and peripheral nervous systems, as well as in chromaffin cells of the adrenal medulla.

특이성

Dopamine beta hydroxylase (DBH).

면역원

Purified bovine DBH.

애플리케이션

Anti-Dopamine β Hydroxylase Antibody, clone 4F10.2 detects level of Dopamine β Hydroxylase & has been published & validated for use in IH, IH(P) & IF.

Indirect immunofluorescence:
1:300-1:500 dilution of a previous lot was used on rat brain tissue.

Optimal working dilutions must be determined by the end user.

Immunohistochemistry(paraffin):
Representative images from a previous lot.

Optimal Staining With Citrate Buffer, pH 6.0, Epitope Retrieval: Rat Spinal Cord

품질

Immunohistochemistry(paraffin): Dopamine β Hydroxylase (cat. # MAB308) staining pattern/morphology in rat spinal cord. Tissue was pretreated with Citrate, pH 6.0. This lot of antibody was diluted to 1:300, using IHC-Select Detection with HRP-DAB. Immunoreactivity is seen predominantly expressed in the dorsal root ganglia (DRG).

Optimal Staining With Citrate Buffer, pH 6.0, Epitope Retrieval: Rat Spinal Cord

결합

Replaces: MAB394-250UG; MAB394-100UG; MAB394-25UG

물리적 형태

Ascites mouse monoclonal IgG1 in liquid with 0.1% sodium azide.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable